A lytic phage to control multidrug-resistant avian pathogenic Escherichia coli (APEC) infection

被引:10
作者
Yao, Lan [1 ,2 ]
Bao, Yinli [3 ]
Hu, Jiangang [1 ]
Zhang, Beibei [1 ]
Wang, Zhiyang [1 ]
Wang, Xinyu [1 ]
Guo, Weiqi [1 ]
Wang, Di [1 ]
Qi, Jingjing [1 ]
Tian, Mingxing [1 ]
Bao, Yanqing [1 ]
Li, Haihua [2 ]
Wang, Shaohui [1 ,2 ]
机构
[1] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai, Peoples R China
[2] Tianjin Agr Univ, Coll Anim Sci & Vet Med, Tianjin Key Lab Agr Anim Breeding & Hlth Husb, Tianjin, Peoples R China
[3] Longyan Univ, Engn Res Ctr Prevent & Control Anim Original Zoono, Coll Life Sci, Longyan, Fujian, Peoples R China
来源
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY | 2023年 / 13卷
基金
上海市自然科学基金; 中国国家自然科学基金;
关键词
avian pathogenic Escherichia coli; multidrug-resistant; bacteriophage; characteristics; biofilm; ANTIBIOTIC-RESISTANCE; BACTERIOPHAGE; CHALLENGES; THERAPY;
D O I
10.3389/fcimb.2023.1253815
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The inappropriate use of antibiotics has led to the emergence of multidrug-resistant strains. Bacteriophages (phages) have gained renewed attention as promising alternatives or supplements to antibiotics. In this study, a lytic avian pathogenic Escherichia coli (APEC) phage designated as PEC9 was isolated and purified from chicken farm feces samples. The morphology, genomic information, optimal multiplicity of infection (MOI), one-step growth curve, thermal stability, pH stability, in vitro antibacterial ability and biofilm formation inhibition ability of the phage were determined. Subsequently, the therapeutic effects of the phages were investigated in the mice model. The results showed that PEC9 was a member of the siphovirus-like by electron microscopy observation. Biological characterization revealed that it could lyse two serotypes of E. coli, including O1 (9/20) and O2 (6/20). The optimal multiplicity of infection (MOI) of phage PEC9 was 0.1. Phage PEC9 had a latent period of 20 min and a burst period of 40 min, with an average burst size of 68 plaque-forming units (PFUs)/cell. It maintained good lytic activity at pH 3-11 and 4-50 & DEG;C and could efficiently inhibit the bacterial planktonic cell growth and biofilm formation, and reduce bacterial counts within the biofilm, when the MOI was 0.01, 0.1, and 1, respectively. Whole-genome sequencing showed that PEC9 was a dsDNA virus with a genome of 44379 bp and GC content of 54.39%. The genome contains 56 putative ORFs and no toxin, virulence, or resistance-related genes were detected. Phylogenetic tree analysis showed that PEC9 is closely related to E. coli phages vB_EcoS_Zar3M, vB_EcoS_PTXU06, SECphi18, ZCEC10, and ZCEC11, but most of these phages exhibit different gene arrangement. The phage PEC9 could successfully protect mice against APEC infection, including improved survival rate, reduced bacterial loads, and organ lesions. To conclude, our results suggest that phage PEC9 may be a promising candidate that can be used as an alternative to antibiotics in the control of APEC infection.
引用
收藏
页数:11
相关论文
共 50 条
  • [21] The characteristic and potential therapeutic effect of isolated multidrug-resistant Acinetobacter baumannii lytic phage
    Behnam Sisakhtpour
    Arezoo Mirzaei
    Vajihe Karbasizadeh
    Nafiseh Hosseini
    Mehdi Shabani
    Sharareh Moghim
    Annals of Clinical Microbiology and Antimicrobials, 21
  • [22] Bacteriostatic Effects of Yujin Powder and Its Components on Clinical Isolation of Multidrug-Resistant Avian Pathogenic Escherichia coli
    Meng, Jinwu
    Wang, Jinli
    Zhu, Jinyue
    Li, Siya
    Qiu, Tianxin
    Wang, Weiran
    Ding, Jinxue
    Wang, Wenjia
    Liu, Jiaguo
    VETERINARY SCIENCES, 2023, 10 (05)
  • [23] Avian pathogenic Escherichia coli (APEC): current insights and future challenges
    Nawaz, Saqib
    Wang, Zhihao
    Zhang, Yan
    Jia, Yuanzheng
    Jiang, Wei
    Chen, Zhaoguo
    Yin, Huifang
    Huang, Cuiqin
    Han, Xiangan
    POULTRY SCIENCE, 2024, 103 (12)
  • [24] Genomic and Proteomic Characterizations of Sfin-1, a Novel Lytic Phage Infecting Multidrug-Resistant Shigella spp. and Escherichia coli C
    Ahamed, S. K. Tousif
    Roy, Banibrata
    Basu, Utpal
    Dutt, Shanta
    Ghosh, A. N.
    Bandyopadhyay, Boudhayan
    Giri, Nabanita
    FRONTIERS IN MICROBIOLOGY, 2019, 10
  • [25] A Phage Collection Propagated in Escherichia coli K-12 Shows Broad Lytic Activity Against a Diverse Panel of Multidrug-Resistant Isolates
    Kaneko, Tomoyoshi
    Aiba, Yoshifumi
    Miyanaga, Kazuhiko
    Cui, Longzhu
    Tsuneda, Satoshi
    PHAGE-THERAPY APPLICATIONS AND RESEARCH, 2025,
  • [26] Application of a novel lytic Jerseyvirus phage LPSent1 for the biological control of the multidrug-resistant Salmonella Enteritidis in foods
    Al-Hindi, Rashad R.
    Alharbi, Mona G.
    Alotibi, Ibrahim
    Azhari, Sheren A.
    Algothmi, Khloud M.
    Esmael, Ahmed
    FRONTIERS IN MICROBIOLOGY, 2023, 14
  • [27] Comprehensive Assessment of Multidrug-Resistant and Extraintestinal Pathogenic Escherichia coli in Wastewater Treatment Plant Effluents
    Park, Ji-Hyun
    Bae, Kyung-Seon
    Kang, Jihyun
    Yoon, Jeong-Ki
    Lee, Soo-Hyung
    MICROORGANISMS, 2024, 12 (06)
  • [28] The characteristic and potential therapeutic effect of isolated multidrug-resistant Acinetobacter baumannii lytic phage
    Sisakhtpour, Behnam
    Mirzaei, Arezoo
    Karbasizadeh, Vajihe
    Hosseini, Nafiseh
    Shabani, Mehdi
    Moghim, Sharareh
    ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS, 2022, 21 (01)
  • [29] Characterization of a lytic Escherichia coli phage CE1 and its potential use in therapy against avian pathogenic Escherichia coli infections
    Tang, Zhaohui
    Tang, Ning
    Wang, Xinwei
    Ren, Huiying
    Zhang, Can
    Zou, Ling
    Han, Lei
    Guo, Longzong
    Liu, Wenhua
    FRONTIERS IN MICROBIOLOGY, 2023, 14
  • [30] Highly Pathogenic and Multidrug Resistant Avian Pathogenic Escherichia Coli in Free-Range Chickens from Brazil
    Oliveira, E. S.
    Cardozo, M., V
    Borzi, M. M.
    Borges, C. A.
    Guastalli, E. A. L.
    Avila, F. A.
    BRAZILIAN JOURNAL OF POULTRY SCIENCE, 2019, 21 (01)