Leaf physiological and morphological constraints of water-use efficiency in C3 plants

The increasing evaporative demand due to climate change will significantly affect the balance of carbon assimilation and water losses of plants worldwide. The development of crop varieties with improved water-use efficiency (WUE) will be critical for adapting agricultural strategies under predicted future climates. This review aims to summarize the most important leaf morpho-physiological constraints of WUE in C-3 plants and identify gaps in knowledge. From the carbon gain side of the WUE, the discussed parameters are mesophyll conductance, carboxylation efficiency and respiratory losses. The traits and parameters affecting the waterside of WUE balance discussed in this review are stomatal size and density, stomatal control and residual water losses (cuticular and bark conductance), nocturnal conductance and leaf hydraulic conductance. In addition, we discussed the impact of leaf anatomy and crown architecture on both the carbon gain and water loss components of WUE. There are multiple possible targets for future development in understanding sources of WUE variability in plants. We identified residual water losses and respiratory carbon losses as the greatest knowledge gaps of whole-plant WUE assessments. Moreover, the impact of trichomes, leaf hydraulic conductance and canopy structure on plants' WUE is still not well understood. The development of a multi-trait approach is urgently needed for a better understanding of WUE dynamics and optimization. Climate-change-induced evaporative demand will impact carbon assimilation and water losses in plants. Developing water-efficient plant varieties is crucial. This review summarizes leaf morpho-physiological constraints on water-use efficiency (WUE) in C3 plants, highlighting knowledge gaps. The understanding of WUE dynamics needs further exploration of residual water losses, respiratory carbon losses, trichomes, leaf hydraulic conductance, and canopy structure. A multi-trait approach is needed for understanding WUE optimizing in plants.