Limosilactobacillus fermentum 3872 That Produces Class III Bacteriocin Forms Co-Aggregates with the Antibiotic-Resistant Staphylococcus aureus Strains and Induces Their Lethal Damage

被引：7

作者：

Abramov, Vyacheslav M. ^{[1
,2
]}

Kosarev, Igor V. ^{[1
,2
]}

Machulin, Andrey V. ^{[3
]}

Priputnevich, Tatiana V. ^{[2
]}

Deryusheva, Evgenia I. ^{[4
]}

Nemashkalova, Ekaterina L. ^{[4
]}

Chikileva, Irina O. ^{[5
]}

Abashina, Tatiana N. ^{[3
]}

Panin, Alexander N. ^{[1
]}

Melnikov, Vyacheslav G. ^{[6
]}

Suzina, Nataliya E. ^{[3
]}

Nikonov, Ilia N. ^{[7
]}

Selina, Marina V. ^{[7
]}

Khlebnikov, Valentin S. ^{[8
]}

Sakulin, Vadim K. ^{[8
]}

Samoilenko, Vladimir A. ^{[3
]}

Gordeev, Alexey B. ^{[2
]}

Sukhikh, Gennady T. ^{[2
]}

Uversky, Vladimir N. ^{[9
]}

Karlyshev, Andrey V. ^{[10
]}

机构：

[1] Russian State Ctr Anim Feed & Drug Standardizat &, Rosselkhoznadzor Fed State Budgetary Inst, Fed Serv Vet & Phytosanitary Surveillance, Moscow 123022, Russia

[2] Minist Hlth, Kulakov Natl Med Res Ctr Obstet Gynecol & Perinato, Moscow 117997, Russia

[3] Russian Acad Sci, Skryabin Inst Biochem & Physiol Microorganisms, Pushchino Sci Ctr Biol Res, Fed Res Ctr, Pushchino 142290, Russia

[4] Russian Acad Sci, Inst Biol Instrumentat, Pushchino Sci Ctr Biol Res, Fed Res Ctr, Pushchino 142290, Russia

[5] Minist Hlth RF, Blokhin Natl Res Ctr Oncol, Lab Cell Immun, Moscow 115478, Russia

[6] Gabrichevsky Res Inst Epidemiol & Microbiol, Moscow 125212, Russia

[7] Moscow State Acad Vet Med & Biotechnol, Fed State Educ Inst Higher Profess Educ, Moscow 109472, Russia

[8] Inst Immunol Engn, Lyubuchany 142380, Russia

[9] Univ S Florida, Morsani Coll Med, Dept Mol Med, Tampa, FL 33612 USA

[10] Kingston Univ London, Fac Hlth, Dept Biomol Sci, Sci, Kingston Upon Thames KT1 2EE, England

来源：

ANTIBIOTICS-BASEL | 2023年 / 12卷 / 03期

关键词：

L; fermentum; bacteriocin; antibacterial activity; S; aureus; antibiotic resistance; INTRINSICALLY DISORDERED PROTEINS; LIVESTOCK-ASSOCIATED MRSA; RISK-FACTORS; LACTOBACILLUS-SALIVARIUS; CELL-WALL; TRANSMISSION; HUMANS; GROWTH; COAGGREGATION; PREVALENCE;

D O I：

10.3390/antibiotics12030471

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

LF3872 was isolated from the milk of a healthy lactating and breastfeeding woman. Earlier, the genome of LF3872 was sequenced, and a gene encoding unique bacteriocin was discovered. We have shown here that the LF3872 strain produces a novel thermolabile class III bacteriolysin (BLF3872), exhibiting antimicrobial activity against antibiotic-resistant Staphylococcus aureus strains. Sequence analysis revealed the two-domain structural (lysozyme-like domain and peptidase M23 domain) organization of BLF3872. At least 25% residues of this protein are expected to be intrinsically disordered. Furthermore, BLF3872 is predicted to have a very high liquid-liquid phase separation. According to the electron microscopy data, the bacterial cells of LF3872 strain form co-aggregates with the S. aureus 8325-4 bacterial cells. LF3872 produced bacteriolysin BLF3872 that lyses the cells of the S. aureus 8325-4 mastitis-inducing strain. The sensitivity of the antibiotic-resistant S. aureus collection strains and freshly isolated antibiotic-resistant strains was tested using samples from women with lactation mastitis; the human nasopharynx and oral cavity; the oropharynx of pigs; and the cows with a diagnosis of clinical mastitis sensitive to the lytic action of the LF3872 strain producing BLF3872. The co-cultivation of LF3872 strain with various antibiotic-resistant S. aureus strains for 24 h reduced the level of living cells of these pathogens by six log. The LF3872 strain was found to be able to co-aggregate with all studied S. aureus strains. The cell-free culture supernatant of LF3872 (CSLF3872) induced S. aureus cell damage and ATP leakage. The effectiveness of the bacteriolytic action of LF3872 strain did not depend on the origin of the S. aureus strains. The results reported here are important for the creation of new effective drugs against antibiotic-resistant strains of S. aureus circulating in humans and animals.

引用

页数：20

共 110 条

[1] Limosilactobacillus fermentum Strain 3872: Antibacterial and Immunoregulatory Properties and Synergy with Prebiotics against Socially Significant Antibiotic-Resistant Infections of Animals and Humans [J].