A novel imprinted locus on bovine chromosome 18 homologous with human chromosome 16q24.1

被引：1

作者：

Huo, Haonan ^{[1
]}

Zhang, Cui ^{[1
]}

Wang, Kun ^{[3
,4
]}

Wang, Siwei ^{[3
,4
]}

Chen, Weina ^{[5
]}

Zhang, Yinjiao ^{[1
]}

Yu, Wenli ^{[2
]}

Li, Shujing ^{[2
]}

Li, Shijie ^{[1
]}

机构：

[1] Agr Univ Hebei, Coll Life Sci, Baoding, Hebei, Peoples R China

[2] Shijiazhuang Tianquan Elite Dairy Ltd, Shijiazhuang, Hebei, Peoples R China

[3] Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crops, Shijiazhuang, Peoples R China

[4] Key Lab Crop Cultivat Physiol & Green Prod Hebei P, Shijiazhuang, Peoples R China

[5] Hebei Univ, Coll Med Sci, Baoding, Hebei, Peoples R China

来源：

MOLECULAR GENETICS AND GENOMICS | 2024年 / 299卷 / 01期

关键词：

Cow; Genomic imprinting; Chromosome; 18; DNA methylation; Isoform-specific; Monoallelic expression; UNIPARENTAL DISOMY; GENE-EXPRESSION;

D O I：

10.1007/s00438-024-02123-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genomic imprinting is an epigenetic regulation mechanism in mammals resulting in the parentally dependent monoallelic expression of genes. Imprinting disorders in humans are associated with several congenital syndromes and cancers and remain the focus of many medical studies. Cattle is a better model organism for investigating human embryo development than mice. Imprinted genes usually cluster on chromosomes and are regulated by different methylation regions (DMRs) located in imprinting control regions that control gene expression in cis. There is an imprinted locus on human chromosome 16q24.1 associated with congenital lethal developmental lung disease in newborns. However, genomic imprinting on bovine chromosome 18, which is homologous with human chromosome 16 has not been systematically studied. The aim of this study was to analyze the allelic expressions of eight genes (CDH13, ATP2C2, TLDC1, COTL1, CRISPLD2, ZDHHC7, KIAA0513, and GSE1) on bovine chromosome 18 and to search the DMRs associated gene allelic expression. Three transcript variants of the ZDHHC7 gene (X1, X2, and X5) showed maternal imprinting in bovine placentas. In addition, the monoallelic expression of X2 and X5 was tissue-specific. Five transcripts of the KIAA0513 gene showed tissue- and isoform-specific monoallelic expression. The CDH13, ATP2C2, and TLDC1 genes exhibited tissue-specific imprinting, however, COTL1, CRISLPLD2, and GSE1 escaped imprinting. Four DMRs, established after fertilization, were found in this region. Two DMRs were located between the ZDHHC7 and KIAA0513 genes, and two were in exon 1 of the CDH13 and ATP2C2 genes, respectively. The results from this study support future studies on the molecular mechanism to regulate the imprinting of candidate genes on bovine chromosome 18.

引用

页数：12

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