Specific Fluorescent Probes for Imaging DNA in Cell-Free Solution and in Mitochondria in Living Cells

被引：6

作者：

Efimova, Anna S. ^{[1
,2
]}

Ustimova, Mariya A. ^{[1
]}

Chmelyuk, Nelly S. ^{[3
]}

Abakumov, Maxim A. ^{[3
]}

Fedorov, Yury V. ^{[1
]}

Fedorova, Olga A. ^{[1
,2
]}

机构：

[1] Russian Acad Sci, AN Nesmeyanov Inst Organoelement Cpds, Lab Photoact Supramol Syst, Vavilova Str 28, Moscow 119334, Russia

[2] Dmitry Mendeleev Univ Chem Technol Russia, Dept Technol Fine Organ Synth & Chem Dyes, Miusskaya Sqr 9, Moscow 125047, Russia

[3] Pirogov Russian Natl Res Med Univ, Dept Med Nanobiotechnoil, Ostrovityanova Str 1, Moscow 117997, Russia

来源：

BIOSENSORS-BASEL | 2023年 / 13卷 / 07期

基金：

俄罗斯科学基金会;

关键词：

styryl dyes; fluorescence probe; DNA binding; mitochondrial selectivity; DYES; BINDING;

D O I：

10.3390/bios13070734

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

New styryl dyes consisting of N-methylpyridine or N-methylquinoline scaffolds were synthesized, and their binding affinities for DNA in cell-free solution were studied. The replacement of heterocyclic residue from the pyridine to quinoline group as well as variation in the phenyl part strongly influenced their binding modes, binding affinities, and spectroscopic responses. Biological experiments showed the low toxicity of the obtained dyes and their applicability as selective dyes for mitochondria in living cells.

引用

页数：13

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