Novel amphiphilic hydroxyethyl starch-based nanoparticles loading camptothecin exhibit high anticancer activity in HepG2 cells and zebrafish

被引:9
作者
Wang, Lizhen [1 ,2 ]
Liu, Xiaolan [3 ]
Zhang, Changqing [4 ]
Chen, Xiqiang [2 ]
Sheng, Wenlong [2 ]
Li, Peihai [2 ]
Qin, Dawei [1 ,5 ]
Wang, Fang [6 ]
机构
[1] Qilu Univ Technol, Shandong Acad Sci, State Key Lab Biobased Mat & Green Papermaking, Jinan 250353, Peoples R China
[2] Qilu Univ Technol, Biol Inst, Shandong Acad Sci, Jinan 250103, Peoples R China
[3] Jinan Author Hosp, Jinan 250001, Peoples R China
[4] Shandong Prov Maternal & Child Hlth Care Hosp, Key Lab Birth Regulat & Control Technol, Natl Hlth Commiss China, Jinan 250014, Peoples R China
[5] Qilu Univ Technol, Shandong Acad Sci, Sch Chem & Chem Engn, Jinan 250353, Peoples R China
[6] Liaocheng Inst Prod Qual Supervis & Inspect, Liaocheng 252022, Peoples R China
关键词
Nanoparticle; Drug delivery system; Hydroxyethyl starch; Anticancer; Zebrafish; MODIFIED BERBERINE DERIVATIVES; DRUG-DELIVERY; POLYMERIC MICELLES; AMINO-ACID; L-LEUCINE; TEMPERATURE; CONJUGATE; NANOMEDICINE; CARRIER; MODEL;
D O I
10.1016/j.colsurfb.2023.113215
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Camptothecin is a naturally occurred anticancer drug but exhibits limitations including poor aqueous solubility, low bioavailability, and high level of adverse drug reactions on normal organs. To overcome these problems, this paper developed a novel amphiphilic Lau-Leu-HES carrier using hydroxyethyl starch, lauric acid, and L-leucine as starting materials. The carrier was successfully applied to prepare Lau-Leu-HES nanoparticles loading camptothecin. The drug loading efficiency and encapsulation efficiency of the nanoparticles were calculated to be 29.04% and 81.85%, respectively. The nanoparticles exhibited high zeta potential (-15.51 mV) and small hydrodynamic diameter (105.4 nm). Camptothecin in nanoparticles could be rapidly released under acidic condition (pH = 4.5), thereby indicating the high sensitivity under cancer microenvironments. Anticancer investigation revealed that the nanoparticles could inhibit the proliferation of HepG2 cells in vitro. Compared with commercial available drug doxorubicin, the nanoparticles could significantly inhibit the expression of krasv12 oncogene in transgenic Tg (EGFP-krasV12) zebrafish. These results indicate that the camptothecin-loaded Lau-Leu-HES nanoparticles are expected to be a potential candidate for cancer therapy.
引用
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页数:10
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