An elucidative study of the anti-biofilm effect of selenium nanoparticles (SeNPs) on selected biofilm producing pathogenic bacteria: A disintegrating effect of SeNPs on bacteria

被引：20

作者：

Ullah, Asad ^{[1
,2
,3
]}

Mirani, Zulfiqar Ali ^{[3
]}

Binbin, Si ^{[1
,2
]}

Wang, Fenghuan ^{[1
,2
]}

Chan, Malik Wajid Hussain ^{[4
]}

Aslam, Sadar ^{[5
]}

Yonghong, Liao ^{[1
,2
]}

Hasan, Najmul ^{[6
]}

Naveed, Muhammad ^{[1
,2
]}

Hussain, Shafqat ^{[7
]}

Khatoon, Zakia ^{[3
,8
]}

机构：

[1] Beijing Technol & Business Univ BTBU, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100048, Peoples R China

[2] Beijing Technol & Business Univ BTBU, Sch Light Ind, Beijing 100048, Peoples R China

[3] Pakistan Council Sci & Ind Res Labs Complex, Food & Marine Resources Res Ctr, Karachi 75280, Pakistan

[4] Fed Urdu Univ Arts Sci & Technol, Fac Sci, Dept Chem, Gulshan e Iqbal Campus, Karachi 75300, Pakistan

[5] Univ Baltistan, Dept Biol Sci, Skardu, Pakistan

[6] Hankuk Univ Foreign Studies, Dept Elect Phys, Seoul, South Korea

[7] Univ Baltistan, Dept Chem, Skardu, Pakistan

[8] Univ Karachi, Inst Marine Sci, Karachi 75270, Pakistan

来源：

PROCESS BIOCHEMISTRY | 2023年 / 126卷

基金：

中国国家自然科学基金;

关键词：

Bacillus subtilis BSN313; Selenium nanoparticles; Anti-biofilm; Disintegrating effect; Atomic force spectroscopy; Zeta potential; ATOMIC-FORCE MICROSCOPY; PSEUDOMONAS-AERUGINOSA; STAPHYLOCOCCUS-AUREUS; CELL-SURFACE; ESCHERICHIA-COLI; AGAROSE GELS; DIFFUSION; BEHAVIOR; GROWTH; CHARGE;

D O I：

10.1016/j.procbio.2022.12.031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, the antibacterial and anti-biofilm activity of selenium nanoparticles (SeNPs) produced by Bacillus subtilis BSN313 was investigated. Agar diffusion and microdilution methods did not reveal significant antibacterial activity of SeNPs against P. aeruginosa ATCC 9027, S. typhi ATCC 14028, and S. aureus ATCC 25923 due to the larger size of SeNPs and higher electrophoretic mobility, zeta potential, and/or poor diffusion properties in agar solids. Therefore, the agar overlay method was introduced, and significant antibacterial ac-tivity was observed with this method at concentrations (SeNPs) of 200 mu g/mL, 150 mu g/mL, and 150 mu g/mL against P. aeruginosa ATCC 9027, S. typhi ATCC 14028, and S. aureus ATCC 25923, respectively. Atomic force spectroscopy (AFM) results, elucidated the disintegrating effect of SeNPs against Gram-negative (P. aeruginosa ATCC 9027) and Gram-positive (S. aureus ATCC 25923) pathogens. The disintegrating effect of SeNPs was caused by the destruction of the bacterial cell (biofilm producing) by rupturing the cell membrane. The hydrophobicity and zeta potential of the planktonic and biofilm populations were measured. Higher values of hydrophobicity and zeta potential heterogeneity were found for the biofilm population than for the planktonic population. The SeNPs showed significant anti-biofilm activity against biofilm-producing pathogenic strains. The ability of SeNPs to disperse biofilms was greatest at a concentration of 700 mu g/mL within 15 min against P. aeruginosa ATCC 9027 (85.7%), S. typhi ATCC 14028 (89.6%), and S. aureus ATCC 25923 (78.3%). While the positive control (ascorbic acid, 32 mu g/mL) showed an average dispersion of 95.1% against the biofilms of the strains studied within 90 min. Based on these results, we propose SeNPs as suitable candidates for the treatment of biofilm-producing pathogens.

引用

页码：98 / 107

页数：10

共 86 条

[1] Synthesis of Selenium Nanoparticles Using Probiotic Bacteria Lactobacillus acidophilus and Their Enhanced Antimicrobial Activity Against Resistant Bacteria [J].