Cytogenetics of the Hybridogenetic Frog Pelophylax grafi and Its Parental Species Pelophylax perezi

被引:2
作者
Dudzik, Anna [1 ]
Dedukh, Dmitrij [2 ]
Crochet, Pierre-Andre [3 ]
Rozenblut-Koscisty, Beata [1 ]
Rybka, Hanna [1 ]
Doniol-Valcroze, Paul [3 ]
Choleva, Lukas [4 ,5 ]
Ogielska, Maria [1 ]
Chmielewska, Magdalena [1 ]
机构
[1] Univ Wroclaw, Fac Biol Sci, Dept Evolutionary Biol & Conservat Vertebrates, Amphibian Biol Grp, Wroclaw, Poland
[2] Czech Acad Sci, Inst Anim Physiol & Genet, Lab Nonmendelian Evolut, Libechov, Czech Republic
[3] Univ Montpellier, EPHE, IRD, CEFE,CNRS, Montpellier, France
[4] Czech Acad Sci, Inst Anim Physiol & Genet, Lab Fish Genet, Libechov, Czech Republic
[5] Univ Ostrava, Fac Sci, Dept Biol & Ecol, Ostrava, Czech Republic
关键词
hybridogenesis; karyotype; Pelophylax grafi; Pelophylax perezi; fluorescent in situ hybridization; comparative genomic hybridization; HYBRID RANA-ESCULENTA; WATER FROGS; GENETIC DIVERSITY; AMPHIBIA; KARYOTYPE; LESSONAE; LAMPBRUSH; RIDIBUNDA; PATTERNS; COMPLEX;
D O I
10.1093/gbe/evad215
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Hybrid taxa from the genus Pelophylax can propagate themselves in a modified way of sexual reproduction called hybridogenesis ensuring the formation of clonal gametes containing the genome of only one parental (host) species. Pelophylax grafi from South-Western Europe is a hybrid composed of P. ridibundus and P. perezi genomes and it lives with a host species P. perezi (P-G system). Yet it is unknown, whether non-Mendelian inheritance is fully maintained in such populations. In this study, we characterize P. perezi and P. grafi somatic karyotypes by using comparative genomic hybridization, genomic in situ hybridization, fluorescent in situ hybridization, and actinomycin D-DAPI. Here, we show the homeology of P. perezi and P. grafi somatic karyotypes to other Pelophylax taxa with 2n = 26 and equal contribution of ridibundus and perezi chromosomes in P. grafi which supports F1 hybrid genome constitution as well as a hemiclonal genome inheritance. We show that ridibundus chromosomes have larger regions of interstitial (TTAGGG)n repeats flanking the nucleolus organizing region on chromosome no. 10 and a high quantity of AT pairs in the centromeric regions. In P. perezi, we found species-specific sequences in metaphase chromosomes and marker structures in lampbrush chromosomes. Pericentromeric RrS1 repeat sequence was present in perezi and ridibundus chromosomes, but the blocks were stronger in ridibundus. Various cytogenetic techniques applied to the P-G system provide genome discrimination between ridibundus and perezi chromosomal sets. They could be used in studies of germ-line cells to explain patterns of clonal gametogenesis in P. grafi and broaden the knowledge about reproductive strategies in hybrid animals.
引用
收藏
页数:15
相关论文
共 59 条
[1]   Phylogeographic patterns of genetic diversity in eastern Mediterranean water frogs were determined by geological processes and climate change in the Late Cenozoic [J].
Akin, Cigdem ;
Bilgin, C. Can ;
Beerli, Peter ;
Westaway, Rob ;
Ohst, Torsten ;
Litvinchuk, Spartak N. ;
Uzzell, Thomas ;
Bilgin, Metin ;
Hotz, Hansjuerg ;
Guex, Gaston-Denis ;
Ploetner, Joerg .
JOURNAL OF BIOGEOGRAPHY, 2010, 37 (11) :2111-2124
[2]  
BERGER L, 1973, Journal of Herpetology, V7, P1, DOI 10.2307/1562822
[3]   Two rare aneutriploids in the unisexual Ambystoma (Amphibia, Caudata) identified by GISH indicating two different types of meiotic errors [J].
Bi, K. ;
Bogart, J. P. ;
Fu, J. .
CYTOGENETIC AND GENOME RESEARCH, 2007, 119 (1-2) :127-130
[4]   Genetic and Genomic Interactions of Animals with Different Ploidy Levels [J].
Bogart, J. P. ;
Bi, K. .
CYTOGENETIC AND GENOME RESEARCH, 2013, 140 (2-4) :117-136
[5]   Unisexual salamanders (genus Ambystoma) present a new reproductive mode for eukaryotes [J].
Bogart, James P. ;
Bi, Ke ;
Fu, Jinzong ;
Noble, Daniel W. A. ;
Niedzwiecki, John .
GENOME, 2007, 50 (02) :119-136
[6]   LAMPBRUSH AND MITOTIC CHROMOSOMES OF THE HEMICLONALLY REPRODUCING HYBRID RANA-ESCULENTA AND ITS PARENTAL SPECIES [J].
BUCCI, S ;
RAGGHIANTI, M ;
MANCINO, G ;
BERGER, L ;
HOTZ, H ;
UZZELL, T .
JOURNAL OF EXPERIMENTAL ZOOLOGY, 1990, 255 (01) :37-56
[7]  
Callan H.G., 1986, LAMPBRUSH CHROMOSOME
[8]   New evidence for parallel evolution of colour patterns in Malagasy poison frogs (Mantella) [J].
Chiari, Y ;
Vences, M ;
Vieites, DR ;
Rabemananjara, F ;
Bora, P ;
Ravoahangimalala, OR ;
Meyer, A .
MOLECULAR ECOLOGY, 2004, 13 (12) :3763-3774
[9]   Genome elimination from the germline cells in diploid and triploid male water frogs Pelophylax esculentus [J].
Chmielewska, Magdalena ;
Kazmierczak, Mikolaj ;
Rozenblut-Koscisty, Beata ;
Kolenda, Krzysztof ;
Dudzik, Anna ;
Dedukh, Dmitrij ;
Ogielska, Maria .
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 2022, 10
[10]   The programmed DNA elimination and formation of micronuclei in germ line cells of the natural hybridogenetic water frog Pelophylax esculentus [J].
Chmielewska, Magdalena ;
Dedukh, Dmitry ;
Haczkiewicz, Katarzyna ;
Rozenblut-Koscisty, Beata ;
Kazmierczak, Mikolaj ;
Kolenda, Krzysztof ;
Serwa, Ewa ;
Pietras-Lebioda, Agnieszka ;
Krasikova, Alla ;
Ogielska, Maria .
SCIENTIFIC REPORTS, 2018, 8