Plastid terminal oxidase (PTOX) protects photosystem I and not photosystem II against photoinhibition in Arabidopsis thaliana and Marchantia polymorpha

被引:5
作者
Messant, Marine [1 ]
Hani, Umama [1 ]
Lai, Thanh-Lan [1 ]
Wilson, Adjele [1 ]
Shimakawa, Ginga [1 ,2 ]
Krieger-Liszkay, Anja [1 ]
机构
[1] Univ Paris Saclay, Inst Integrat Biol Cell I2BC, CEA, CNRS, F-91198 Gif Sur Yvette, France
[2] Kwansei Gakuin Univ, Sch Biol & Environm Sci, Dept Biosci, 1 Gakuen Uegahara, Sanda, Hyogo 6691330, Japan
关键词
Arabidopsis thaliana; Marchantia polymorpha; photosynthesis; photoinhibition; regulation; ALTERNATIVE ELECTRON SINKS; LIVERWORT; IMMUTANS; STRESS; CHLORORESPIRATION;
D O I
10.1111/tpj.16520
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The plastid terminal oxidase PTOX controls the oxidation level of the plastoquinone pool in the thylakoid membrane and acts as a safety valve upon abiotic stress, but detailed characterization of its role in protecting the photosynthetic apparatus is limited. Here we used PTOX mutants in two model plants Arabidopsis thaliana and Marchantia polymorpha. In Arabidopsis, lack of PTOX leads to a severe defect in pigmentation, a so-called variegated phenotype, when plants are grown at standard light intensities. We created a green Arabidopsis PTOX mutant expressing the bacterial carotenoid desaturase CRTI and a double mutant in Marchantia lacking both PTOX isoforms, the plant-type and the alga-type PTOX. In both species, lack of PTOX affected the redox state of the plastoquinone pool. Exposure of plants to high light intensity showed in the absence of PTOX higher susceptibility of photosystem I to light-induced damage while photosystem II was more stable compared with the wild type demonstrating that PTOX plays both, a pro-oxidant and an anti-oxidant role in vivo. Our results shed new light on the function of PTOX in the protection of photosystem I and II.
引用
收藏
页码:669 / 678
页数:10
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