Biogenic synthesis of Ag NPs adorned Blueberry fruit extract-modified graphene oxide: Investigation of its anti-gastric cancer effects

An ecofriendly approach for Ag NPs adorned Blueberry fruit extract-modified graphene oxide nanohybrid material has been demonstrated. Initially, the Blueberry fruit was functionalized over the surface of GO. The poly hydroxyl groups of plant phytochemicals facilitated the green reduction of Ag ions over the surface of GO to made the GO@Blueberry/Ag nanocomposite. The nanocomposite was characterized using FT-IR, FE-SEM, EDX, ICP, TEM and XRD analysis. To survey the cytotoxicity and anti-human gastric cancer effects of GO@Blueberry/ Ag nanocomposite, MTT assay was used on the GC1436, AGS, and KATO III cell lines. GO@Blueberry/Ag nanocomposite had very low cell viability and anti-human gastric cancer properties dose-dependently against GC1436, AGS, and KATO III gastric cancer cell lines without any cytotoxicity on the normal cell line. The molecular pathway analysis of GO@Blueberry/Ag nanocomposite-treated cells revealed that nanoparticles enhance p53 expression, while inhibiting the expression of total and phosphorylated Signal Transducer and Activator Of Transcription 3 (STAT3) in cell lines, suggesting p53 and STAT3 are the main key players behind the biological events provoked by the nanoparticles in human cancer cells. The IC50 of GO@Blueberry/Ag nanocomposite were 85, 59, and 70 & mu;g/mL against GC1436, AGS, and KATO III cell lines, respectively. The best result of anti-human gastric cancer properties of GO@Blueberry/Ag nanocomposite against the above cell lines was seen in the case of the AGS cell line. After doing the clinical trial studies, the recent nanocomposite can be used as a new anti-gastric cancer drug or supplement in human.