The effect of exosomes released from apheresis plateletconcentrates under the impact of gamma irradiation and storage time upon platelet aggregation and hemostasis

被引：1

作者：

Bal, Salih Haldun ^{[1
,2
]}

Sagdilek, Engin ^{[3
]}

Karacay, Mehmet ^{[2
,4
]}

Kizmaz, Muhammed A. ^{[2
,4
]}

Kumas, Levent T. ^{[5
]}

Can, Fatma E. ^{[6
,7
]}

Yildirim, Muzaffer ^{[8
]}

Canavar-Yildirim, Tugce ^{[8
]}

Kosay-Gulkaya, Deniz ^{[2
]}

Yoyen-Ermis, Digdem ^{[2
]}

Budak, Ferah ^{[2
]}

Heper, Yasemin ^{[1
,9
]}

Gursel, Ihsan ^{[8
,10
]}

Oral, Haluk B. ^{[2
,3
]}

机构：

[1] Bursa Uludag Univ, Fac Med, Dr Rasit Durusoy Blood Bank, Bursa, Turkiye

[2] Bursa Uludag Univ, Fac Med, Dept Immunol, Bursa, Turkiye

[3] Bursa Uludag Univ, Fac Med, Dept Biophys, Bursa, Turkiye

[4] Bursa Uludag Univ, Inst Hlth Sci, Dept Med Immunol, Bursa, Turkiye

[5] Bursa Uludag Univ, Inst Hlth Sci, Dept Med Microbiol Microbiol Immunol, Bursa, Turkiye

[6] Bursa Uludag Univ, Inst Hlth Sci, Dept Biostat, Bursa, Turkiye

[7] Izmir Katip Celebi Univ, Fac Med, Dept Biostat, Izmir, Turkiye

[8] Ihsan Dogramaci Bilkent Univ, Fac Sci, Dept Mol Biol & Genet, Thorlab Therapeut Oligodeoxynucleotide Res Lab, Ankara, Turkiye

[9] Bursa Uludag Univ, Fac Med, Dept Infect Dis & Clin Microbiol, Bursa, Turkiye

[10] Izmir Biomed & Genome Ctr, Off Director, Izmir, Turkiye

来源：

BLOOD TRANSFUSION | 2023年 / 21卷 / 03期

基金：

欧盟地平线“2020”;

关键词：

aggregation; exosome; gamma irradiation; hemostasis; platelet concentrates; EXTRACELLULAR VESICLES; QUALITY-CONTROL; HUMAN PLASMA; MICROPARTICLES; INACTIVATION; ACTIVATION; RADIATION;

D O I：

10.2450/2022.006-22

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background -Blood components should be gamma-irradiated (& gamma;-IR) in order to prevent transfusion-associated graft-versus-host disease. The aim of this study is to determine the effect of & Gamma;-IR and storage time on the exosomes released from apheresis platelet concentrates (aPC) and to investigate their impact on the maximum platelet aggregation (MPA) and hemostasis. Materials and methods -Eight units of aPC were included in this study. These were divided into four equal portions. Two portions were irradiated before storage while the other two were not. Thus, irradiated and non-irradiated aPC samples for storage Days 0 (D0) and 5 (D5) were obtained. Exosomes were isolated from these samples using a commercial kit and were evaluated to ascertain their parent cells by flow cytometry. For the following steps, exosomes were pooled according to their features. Pooled exosomes were then used for aggregometry and thromboelastography. Results -Platelet-derived exosome (PD-EX) levels decreased in D5 compared to D0 in NI-aPC, whereas granulocyte-derived exosome (GD-EX) levels increased. Exosome pools had no effect on MPA compared to saline groups. Exosome pools decreased the time to initial fibrin formation (R), whereas they increased the rate of clot formation (& alpha;-angle) and coagulation index (CI) compared to saline groups. Discussion -Storage time and & Gamma;-IR each have almost the opposite effects on PD-EX and GD-EX. Exosomes have no impact on MPA, but enhance the clot strength. The impact of exosomes on aPC quality and effectiveness can be ignored or considered as a positive effect.

引用

页码：257 / 267

页数：11