Association of Variants in Innate Immune Genes TLR4 and TLR5 with Reproductive and Milk Production Traits in Czech Simmental Cattle

被引:0
作者
Novak, Karel [1 ]
Valcikova, Terezie [2 ]
Samake, Kalifa [3 ]
Bjelka, Marek [4 ]
机构
[1] Inst Anim Sci, Dept Genet & Breeding Farm Anim, Pratelstvi 815, Prague 10401, Czech Republic
[2] Czech Univ Life Sci, Dept Genet, Kamycka 129, Prague 16506, Czech Republic
[3] Charles Univ Prague, Dept Genet & Microbiol, Fac Sci, Vinicna 7, Prague 12843, Czech Republic
[4] Breeding Co CHD Impuls, Bohdalec 59255, Czech Republic
关键词
cattle; genotyping; immunity; inflammation; mammary gland; parturition; toll-like receptors; FALSE DISCOVERY RATE; TOLL-LIKE RECEPTORS; DAIRY; DIVERSITY; PARATUBERCULOSIS; POLYMORPHISMS; PREDICTION; MYOMETRIAL; RESISTANCE; INFECTION;
D O I
10.3390/genes15010024
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Bovine genes TLR4 and TLR5, which encode antibacterial toll-like receptors, were screened for polymorphisms in Czech Red Pied (Czech Simmental) cattle to identify variants associated with reproduction, udder health, and milk production traits. Variants were discovered by hybrid resequencing of 164 bulls using HiSeq X-Ten and PacBio technologies and then individually genotyped. Nominal p-values < 0.05 for associations were detected in 18 combinations between 14 polymorphisms and 15 traits using one-way analysis of variance (ANOVA). The TLR4 variants g.610C>T (rs43578094) and g.10310T>G (rs8193072) in reference AC000135.1 were strictly associated with the index of early reproductive disorders and maternal calving ease, respectively, at false discovery rate (FDR) < 0.05. A highly permissive false discovery rate cutoff of 0.6 separated seventeen combinations in both genes comprising eight positives. In the case of the TLR4 variant g.9422T>C (rs8193060), indications were obtained for the association with as many as four reproductive traits: incidence of cystic ovaries, early reproductive disorders, calving ease, and production longevity. The permissive FDR interpretation for the TLR5 data indicated associations with cyst incidence and early reproduction disorders with maternal calving ease. Moreover, three TLR5 polymorphisms correlated with milk production traits. The discrepancy of the observed associations with the predicted impacts of the SNPs on protein function points to the role of haplotypes. Nevertheless, this question should be resolved on a larger scale. The observed associations are endorsed by independent evidence from the published functional roles in other species and by the published QTL mapping data.
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页数:12
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