Structure and Metabolic Activity of the Gut Microbiota in Diarrhea-Predominant Irritable Bowel Syndrome Combined with Functional Dyspepsia

被引:2
作者
Kovaleva, Aleksandra [1 ]
Poluektova, Elena [1 ,2 ]
Maslennikov, Roman [1 ,2 ,3 ]
Zolnikova, Oxana [1 ]
Shifrin, Oleg [1 ]
Kudryavtseva, Anna [4 ]
Krasnov, George [4 ]
Fedorova, Maria [4 ]
Karchevskaya, Anna [1 ]
Ivashkin, Vladimir [1 ]
机构
[1] Sechenov Univ, Dept Internal Med Gastroenterol & Hepatol, Moscow 119991, Russia
[2] Interreg Publ Org Sci Community Promot Clin Study, Moscow 119991, Russia
[3] Moscow Hlth Dept, Consultat & Diagnost Ctr 2, Moscow 107564, Russia
[4] Russian Acad Sci, Engelhardt Inst Mol Biol, Postgenom Res Lab, Moscow 119991, Russia
来源
GASTROINTESTINAL DISORDERS | 2023年 / 5卷 / 03期
关键词
irritable bowel syndrome; functional dyspepsia; short-chain fatty acids; gut microbiota; gut microbiome; CHAIN FATTY-ACIDS; INTESTINAL MICROBIOTA; HEALTHY CONTROLS; DISEASE;
D O I
10.3390/gidisord5030024
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Gut dysbiosis presents in many digestive diseases. The aim of this study is to investigate the composition of the gut microbiota and its metabolic activity in patients with diarrhea-predominant irritable bowel syndrome combined with functional dyspepsia (I + D). This study included 60 patients with I + D and 20 healthy controls. Gut microbiota composition was studied using 16S rRNA gene sequencing. The short-chain fatty acids (SCFAs) spectrum was determined via gas-liquid chromatography. Patients with I + D had an increase in the abundance of Holdemanella, Erysipelotrichaceae, Erysipelotrichales, Prevotellaceae, Agathobacter, Slackia, Lactococcus, Pseudomonadaceae, Stenotrophomonas, Xanthomonadaceae, Rhizobiaceae, Erysipelatoclostridiaceae, Lachnospiraceae, and other taxa in addition to a decrease in the abundance of Frisingicoccus, Ralstonia, Burkholderiaceae, Hungatella, Eisenbergiella, Parabacteroides, Peptostreptococcaceae, Merdibacter, Bilophila, Rikenellaceae, Tannerellaceae, Bacteroidaceae, and Flavonifractor in comparison to controls. Patients with I + D showed significantly higher total SCFA content in feces; increased absolute content of acetic acid, propionic acid, butyric acid, and isoacids; and a significant negative shift in the anaerobic index. The relative levels of the main SCFAs and isoacids in the patient group did not differ significantly from those in the control group. The fecal acetate and isoacid levels correlated with the severity of diarrhea. The fecal butyrate level correlated with the severity of flatulence.
引用
收藏
页码:296 / 309
页数:14
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