Enhanced bidirectional extracellular electron transfer based on biointerface interaction of conjugated polymers-bacteria biohybrid system

The low bacteria loading capacity and low extracellular electron transfer (EET) efficiency are two major bottlenecks restricting the performance of the bioelectrochemical systems from practical applications. Herein, we demonstrated that conjugated polymers (CPs) could enhance the bidirectional EET efficiency through the intimate biointerface interactions of CPs-bacteria biohybrid system. Upon the formation of CPs/bacteria biohybrid, thick and intact CPs-biofilm formed which ensured close biointerface interactions between bacteria-to-bacteria and bacteria-to-electrode. CPs could promote the transmembrane electron transfer through intercalating into the cell membrane of bacteria. Utilizing the CPs-biofilm biohybrid electrode as anode in microbial fuel cell (MFC), the power generation and lifetime of MFC had greatly improved based on accelerated outward EET. Moreover, using the CPs-biofilm biohybrid electrode as cathode in electrochemical cell, the current density was increased due to the enhanced inward EET. Therefore, the intimate biointerface interaction between CPs and bacteria greatly enhanced the bidirectional EET, indicating that CPs exhibit promising applications in both MFC and microbial electrosynthesis.