Melatonin protects retinal tissue damage in streptozotocin-induced aged rats

被引：4
作者：
Atacak, Adem [1 ]
Baltaci, Saltuk Bugra [1 ]
Akgun-Unal, Nilufer [2 ]
Mogulkoc, Rasim [1 ]
Baltaci, Abdulkerim Kasim [1 ]
机构：
[1] Selcuk Univ, Med Fac, Dept Physiol, Konya, Turkiye
[2] Univ Ondokuz Mayis, Fac Med, Dept Biophys, Samsun, Turkiye
来源：
ARCHIVES OF GERONTOLOGY AND GERIATRICS | 2023年 / 112卷
关键词：
Diabetes; Retinal tissue; SIRT1; Oxidative stres; Melatonin; DIABETIC-RETINOPATHY; OXIDATIVE STRESS; NEGATIVE REGULATOR; SIRT1; MATRIX-METALLOPROTEINASE-9; INHIBITION; MECHANISM;
D O I：
10.1016/j.archger.2023.105035
中图分类号：
R592 [老年病学]; C [社会科学总论];
学科分类号：
03 ; 0303 ; 100203 ;
摘要：
Objectives: The aim of this study was to investigate how melatonin administration affects retinal oxidative damage and retinal SIRT1 gene activation in diabetic elderly female rat model. Methods: 16-months-old female rats were used in the study. A total of 24 rats were divided into 4 groups in equal numbers: Group 1. Control, Group 2. Control + Melatonin, Group 3. Diabetes, Group 4. Diabetes + Melatonin. In group 3 and 4 rats, diabetes was induced by intraperitoneal (IP) injection of streptozotocin. Groups 2 and 4 were given ip melatonin for 4 weeks. SIRT-1 gene expression was determined by PCR method and GSH and MDA levels by ELISA in retinal tissue samples taken from animals sacrificed under general anesthesia. Results: In our study, the highest retinal SIRT1 expression values were obtained in the diabetes + melatonin (G4) group. The retinal SIRT1 expression values of the diabetes group (G3) were lower than group 4 and higher than the general control (G1) and control + melatonin (G2) groups. Again in our study, the highest retinal MDA values were obtained in the diabetes group (G3). The highest retinal GSH values were obtained in the Diabetes + melatonin group (G4). Conclusion: The results of our study showed that melatonin supplementation has a protective effect on retinal tissue in a diabetic elderly female rat model. This protective effect of melatonin supplementation occurs by increasing both retinal antioxidant activity and retinal SIRT1 gene expression.
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页数：6
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