Novel immunochromatographic estimation of lamb content in meat products using IgG as biomarker

被引：6

作者：

Hendrickson, Olga D. ^{[1
]}

Zvereva, Elena A. ^{[1
]}

Dzantiev, Boris B. ^{[1
]}

V. Zherdev, Anatoly ^{[1
]}

机构：

[1] Russian Acad Sci, AN Bach Inst Biochem, Res Ctr Biotechnol, Leninsky prospect 33, Moscow 119071, Russia

来源：

JOURNAL OF FOOD COMPOSITION AND ANALYSIS | 2023年 / 116卷

基金：

俄罗斯科学基金会;

关键词：

Immunochromatographic analysis; immunoglobulins as biomarkers; lamb meat; meat adulteration; lamb identification; food security; ENZYME-IMMUNOASSAY; PORK ADULTERATION; TROPONIN-I; QUANTIFICATION; IDENTIFICATION; BINDING; MUSCLE;

D O I：

10.1016/j.jfca.2022.105025

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The aim of this study was to develop a novel immunochromatographic analysis (ICA) for the determination of lamb additives in raw meat and meat products. Sheep immunoglobulins of class G (IgG) served as the molecular identifier. The ICA was implemented in a sandwich format using anti-species antibodies labeled with gold nanoparticles. Sheep IgG was detected with the instrumental detection limit of 60 ng/mL and the cutoff of 10 ng/ mL within 13 min. The high specificity of the ICA towards only sheep IgG was confirmed within the determination of immunoglobulins of other mammals and birds and analysis of extracts from the corresponding sorts of meat. It was shown that the test system can reliably determine 1% lamb additive in chicken meat. The test system was successfully used for lamb identification in dumplings of various compositions. The proposed ICA is a promising approach for the determination of species-specific immunoglobulins as biomarkers of composition and adulteration of meat products.

引用

页数：7