A Metal Organic Framework-Based Light Scattering ELISA for the Detection of Staphylococcal Enterotoxin B

被引:1
|
作者
Mao, Kai [1 ]
Tian, Lili [1 ]
Luo, Yujie [1 ]
Li, Qian [2 ]
Chen, Xi [1 ]
Zhan, Lei [2 ]
Li, Yuanfang [1 ]
Huang, Chengzhi [2 ]
Zhen, Shujun [1 ]
机构
[1] Southwest Univ, Minist Educ, Coll Chem & Chem Engn, Key Lab Luminescence Anal & Mol Sensing, Chongqing 400715, Peoples R China
[2] Southwest Univ, Coll Pharmaceut Sci, Chongqing Sci & Technol Bur, Key Lab Luminescent & Real Time Analyt Chem, Chongqing 400715, Peoples R China
关键词
ELISA; light scattering; metal organic framework; ZIF-8; staphylococcal enterotoxin B; PARTICLES; DNA; BIOSENSOR; ZIF-8;
D O I
10.3390/chemosensors11080453
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Enzyme-linked immunosorbent assay (ELISA) is one of the most commonly used method for the detection of staphylococcal enterotoxin B (SEB), the main protein toxin causing staphylococcal food poisoning. However, the traditional ELISA reaction needs to be stopped by sulfuric acid to obtain stable colorimetric signal, and it is easily influenced by a colored sample. In order to address this problem, a new ELISA method using zeolite imidazolate skeleton-8 metal-organic framework (ZIF-8 MOF) as a light scattering (LS) reporter for SEB detection was developed in this work. ZIF-8 MOF has the characteristics of high porosity, large specific surface area, clear pore structure, and adjustable size, which is one of the most representative MOFs constructed from Zn2+ and 2-methylimidazole (2-mIM). The 2-mIM ligand of ZIF-8 exhibited antioxidant activity and can strongly react with H2O2, which could destroy the structure of ZIF-8, resulting in the obvious decrease in LS intensity. We combined this specific reaction with the sandwich immune reaction to construct the LS ELISA method for the successful detection of SEB. This method is more reliable than commercial tests kits for the detection of colored samples, and it is simple, sensitive, and selective, and has great potential in the detection of other toxins by simply changing the corresponding recognition units.
引用
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页数:13
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