Integrated analysis of the lncRNA-associated ceRNA network in Alzheimer's disease

被引:4
作者
Cai, Hong-Yan [1 ,2 ,3 ]
Chen, Si-Ru [1 ]
Wang, Yu [1 ]
Jiao, Juan-Juan [4 ]
Qiao, Jun [2 ,5 ]
Holscher, Christian [6 ]
Wang, Zhao-Jun [2 ,3 ,4 ]
Zhang, Sheng-Xiao [2 ,5 ]
Wu, Mei-Na [2 ,3 ,4 ]
机构
[1] Shanxi Med Univ, Dept Microbiol & Immunol, Taiyuan, Peoples R China
[2] Shanxi Med Univ, Key Lab Cellular Physiol, Minist Educ, Taiyuan, Peoples R China
[3] Key Lab Cellular Physiol, Taiyuan, Shanxi, Peoples R China
[4] Shanxi Med Univ, Dept Physiol, Taiyuan, Peoples R China
[5] Shanxi Med Univ, Dept Rheumatol, Hosp 2, Taiyuan, Shanxi, Peoples R China
[6] Henan Univ Chinese Med, Acad Chinese Med Sci, Zhengzhou, Peoples R China
基金
中国国家自然科学基金;
关键词
Alzheimer's disease; lncRNA; mRNA; ceRNA; GO; KEGG; CYCLASE-ACTIVATING POLYPEPTIDE; GENE-EXPRESSION; PATHOGENESIS; SYSTEM; MET;
D O I
10.1016/j.gene.2023.147484
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Alzheimer's disease (AD) is a progressive neurodegenerative disease that worsens with age. Long non-coding RNAs (lncRNAs) dysregulation and its associated competing endogenous RNA (ceRNA) network have a potential connection with the occurrence and development of AD. A total of 358 differentially expressed genes (DEGs) were screened via RNA sequencing, including 302 differentially expressed mRNAs (DEmRNAs) and 56 differential expressed lncRNAs (DElncRNAs). Anti-sense lncRNA is the main type of DElncRNA, which plays a major role in the cis and trans regulation. The constructed ceRNA network consisted of 4 lncRNAs (NEAT1, LINC00365, FBXL19-AS1, RAI1-AS1719) and 4 microRNAs (miRNAs) (HSA-Mir-27a-3p, HSA-Mir-20b-5p, HSA-Mir-17-5p, HSA-Mir-125b-5p), and 2 mRNAs (MKNK2, F3). Functional enrichment analysis revealed that DEmRNAs are involved in related biological functions of AD. The co-expressed DEmRNAs (DNAH11, HGFAC, TJP3, TAC1, SPTSSB, SOWAHB, RGS4, ADCYAP1) of humans and mice were screened and verified by real-time quantitative polymerase chain reaction (qRT-PCR). In this study, we analyzed the expression profile of human AD-related lncRNA genes, constructed a ceRNA network, and performed functional enrichment analysis of DEmRNAs between human and mice. The obtained gene regulatory networks and target genes can be used to further analyze AD-related pathological mechanisms to optimize AD diagnosis and treatment.
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页数:10
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