Label-free and dynamic monitoring of cell evolutions using wavelength-multiplexing surface plasmon resonance holographic microscopy

被引:4
作者
Dai, Siqing [1 ,2 ]
Mi, Jingyu [1 ,2 ]
Dou, Jiazhen [1 ,2 ]
Shi, Wenpu [3 ]
Zhang, Jiwei [1 ,2 ]
Zhao, Jianlin [1 ,2 ]
机构
[1] Northwestern Polytech Univ, Sch Phys Sci & Technol, Key Lab Light Field Manipulat & Informat Acquisit, Minist Ind & Informat Technol, Xian 710129, Peoples R China
[2] Northwestern Polytech Univ, Sch Phys Sci & Technol, Shaanxi Key Lab Opt Informat Technol, Xian 710129, Peoples R China
[3] Northwestern Polytech Univ, Sch Life Sci, Key Lab Space Biosci & Biotechnol, Xian 710072, Shaanxi, Peoples R China
基金
中国国家自然科学基金;
关键词
REFRACTIVE-INDEX; PHASE; AMPLITUDE; ADHESION; DISTANCE; IMAGES; BIO;
D O I
10.1364/BOE.486467
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Dynamic characterizations of intracellular variations and cell-substrate interactions under different external environments are critical to study cell behaviors and exploring biological applications. However, techniques that are capable of dynamically and simultaneously measuring multiple parameters of living cells in a wide-field manner have rarely been reported. Here, we present a wavelength-multiplexing surface plasmon resonance holographic microscopy which allows wide-field, simultaneous, and dynamic measurements of cell parameters, including cell-substrate distance and cytoplasm refractive index (RI). We use two lasers of 632.8 nm and 690 nm as light sources. Two beam splitters are employed in the optical setup to separately adjust the incident angle of two light beams. Then, surface plasmon resonance (SPR) can be excited for each wavelength under SPR angles. We demonstrate the advances of the proposed apparatus by systematically studying the cell responses to osmotic pressure stimuli from the environmental medium at the cell-substrate interface. The SPR phase distributions of the cell are firstly mapped at two wavelengths, then the cell-substrate distance and cytoplasm RI are retrieved using a demodulation method. Based on phase response differences between two wavelengths and monotonic changes of SPR phase with cell parameters, cell-substrate distance, and cytoplasm RI can be determined simultaneously using an inverse algorithm. This work affords a new optical measurement technique to dynamically characterize cell evolutions and investigate cell properties in various cellular activities. It may become a useful tool in the bio-medical and bio-monitoring areas.
引用
收藏
页码:2028 / 2039
页数:12
相关论文
共 42 条
[1]   Plasmonic-based impedance microspectroscopy of optically heterogeneous samples [J].
Abayzeed, Sidahmed A. .
BIOMEDICAL OPTICS EXPRESS, 2020, 11 (11) :6168-6180
[2]   Uncovering phase maps from surface plasmon resonance images: Towards a sub-wavelength resolution [J].
Argoul, Francoise ;
Roland, Thibault ;
Fahys, Audrey ;
Berguiga, Lotfi ;
Elezgaray, Juan .
COMPTES RENDUS PHYSIQUE, 2012, 13 (08) :800-814
[3]   Amplitude and phase images of cellular structures with a scanning surface plasmon microscope [J].
Berguiga, L. ;
Roland, T. ;
Monier, K. ;
Elezgaray, J. ;
Argoul, F. .
OPTICS EXPRESS, 2011, 19 (07) :6571-6586
[4]   Time-lapse scanning surface plasmon microscopy of living adherent cells with a radially polarized beam [J].
Berguiga, Lotfi ;
Streppa, Laura ;
Boyer-Provera, Elise ;
Martinez-Torres, Cristina ;
Schaeffer, Laurent ;
Elezgaray, Juan ;
Arneodo, Alain ;
Argoul, Francoise .
APPLIED OPTICS, 2016, 55 (06) :1216-1227
[5]   Advances in Surface Plasmon Resonance Imaging and Microscopy and Their Biological Applications [J].
Bockova, Marketa ;
Slaby, Jiri ;
Springer, Tomas ;
Homola, Jiri .
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 12, 2019, 12 :151-176
[6]   Application of total internal reflection fluorescence microscopy to study cell adhesion to biomaterials [J].
Burmeister, JS ;
Olivier, LA ;
Reichert, WM ;
Truskey, GA .
BIOMATERIALS, 1998, 19 (4-5) :307-325
[7]   Long range surface plasmon resonance for increased sensitivity in living cell biosensing through greater probing depth [J].
Chabot, Vincent ;
Miron, Yannick ;
Grandbois, Michel ;
Charette, Paul G. .
SENSORS AND ACTUATORS B-CHEMICAL, 2012, 174 :94-101
[8]   Label-free surface plasmon resonance cytosensor for breast cancer cell detection based on nano-conjugation of monodisperse magnetic nanoparticle and folic acid [J].
Chen, Hongxia ;
Hou, Yafei ;
Ye, Zonghuang ;
Wang, Haiyan ;
Koh, Kwangnak ;
Shen, Zhongming ;
Shu, Yongqian .
SENSORS AND ACTUATORS B-CHEMICAL, 2014, 201 :433-438
[9]   Dual-wavelength surface plasmon resonance holographic microscopy for simultaneous measurements of cell-substrate distance and cytoplasm refractive index [J].
Dai, Siqing ;
Mi, Jingyu ;
Dou, Jiazhen ;
Yu, Tongyao ;
Zhang, Mengmeng ;
Di, Jianglei ;
Zhang, Jiwei ;
Zhao, Jianlin .
OPTICS LETTERS, 2022, 47 (09) :2306-2309
[10]   Optical tweezers integrated surface plasmon resonance holographic microscopy for characterizing cell-substrate interactions under noninvasive optical force stimuli [J].
Dai, Siqing ;
Mi, Jingyu ;
Dou, Jiazhen ;
Lu, Hua ;
Dong, Chen ;
Ren, Li ;
Zhao, Rong ;
Shi, Wenpu ;
Zhang, Nu ;
Zhou, Yidan ;
Zhang, Jiwei ;
Di, Jianglei ;
Zhao, Jianlin .
BIOSENSORS & BIOELECTRONICS, 2022, 206