Anticancer activity of Zinc-Sodium alginate-Polyethylene glycol- Brucine nanocomposite in gallbladder cancer NOZ cells via modulation of apoptosis and P13K/mTOR pathway

Background: Gallbladder cancer (GBC) is the sixth most predominant gastrointestinal tumor worldwide. Malignant tumors with distinctive biological characteristics, like the human GBC, are incredibly aggressive and challenging to diagnose and treat. Objective: The aim of the present study is to explore the in vitro anticancer properties of ZAP-Brucine nanocomposites (ZAP-Bru NCs) on gallbladder cancer cells. Methodology: The cytotoxicity of the ZAP-Bru NCs at concentrations ranging from 10 to 60 mg/ mL on the NOZ cells was assessed by an MTT study. The IC50 value of ZAP-Bru NCs was found to be 5 mg/ml, which indicates that it has 50% inhibitory effects on NOZ cells than other doses. Several fluorescent staining approaches, such as dual staining and rhodamine-123 techniques, were applied to investigate the apoptosis and MMP levels in the treated NOZ cells. The generation of ROS in the treated NOZ cells was examined by DCFH-DA staining. The modifications in the expressions of inflammatory genes like NF-KB, TNF-a, IL-6, COX-2, and IL-6 in the treated NOZ cells were examined using the respective kits.