Synthesis, characterization, and in vitro anti-tumor activity studies of the hyaluronic acid-mangiferin-methotrexate nanodrug targeted delivery system

被引:9
|
作者
Wang, Haojue [1 ]
Shao, Wanfei [2 ]
Lu, Xianyi [1 ]
Gao, Chunxia [2 ]
Fang, Ling [3 ]
Yang, Xiaojun [4 ]
Zhu, Peizhi [2 ]
机构
[1] Wuxi Xishan Peoples Hosp Jiangsu Prov, Dept Obstet & Gynecol, Wuxi 214105, Peoples R China
[2] Yangzhou Univ, Sch Chem & Chem Engn, Yangzhou 225002, Peoples R China
[3] Wuxi Xishan Peoples Hosp, Dept Dermatol, Wuxi 214105, Jiangsu, Peoples R China
[4] Soochow Univ, Affiliated Hosp 1, 188 Shizi Rd, Suzhou 215006, Jiangsu, Peoples R China
关键词
Hyaluronic acid; CD44; receptor; Methotrexate; Mangiferin; Nanoparticles; DRUG-DELIVERY; CANCER; ANTICANCER; NANOPARTICLES; THERAPEUTICS; DENDRIMERS; DESIGN;
D O I
10.1016/j.ijbiomac.2023.124208
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In this study, to increase the accumulation of MTX in the tumor site and reduce the toxicity to normal tissues by MA, a novel nano-drug delivery system comprised of hyaluronic acid (HA)-mangiferin (MA)-methotrexate (MTX) (HA-MA-MTX) was developed by a self-assembly strategy. The advantage of the nano-drug delivery system is that MTX can be used as a tumor-targeting ligand of the folate receptor (FA), HA can be used as another tumor -targeting ligand of the CD44 receptor, and MA serves as an anti-inflammatory agent. 1HNMR and FT-IR re-sults confirmed that HA, MA, and MTX were well coupled together by the ester bond. DLS and AFM images revealed that the size of HA-MA-MTX nanoparticles was about -138 nm. In vitro cell experiments proved that HA-MA-MTX nanoparticles have a positive effect on inhibiting K7 cancer cells while having relatively lower toxicity to normal MC3T3-E1 cells than MTX does. All these results indicated that the prepared HA-MA-MTX nanoparticles can be selectively ingested by K7 tumor cells through FA and CD44 receptor-mediated endocy-tosis, thus inhibiting the growth of tumor tissues and reducing the nonspecific uptake toxicity caused by chemotherapy. Therefore, these self-assembled HA-MA-MTX NPs could be a potential anti-tumor drug delivery system.
引用
收藏
页数:14
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