LC-MS guided isolation of phenolic glycosides from Viburnum luzonicum Rolfe leaves and their α-amylase and α-glucosidase inhibitory activities

被引：11

作者：

Chen, Jia ^{[1
]}

Zhao, Min ^{[1
]}

Zhang, Xiao-Hui ^{[2
]}

Zhao, Chun-Jie ^{[1
]}

Zhao, Zi-Yang ^{[2
]}

Tang, Yi-Yuan ^{[2
]}

Zhou, Hong-Juan ^{[2
]}

Shao, Jian-Hua ^{[2
]}

Zhao, Chun-Chao ^{[2
]}

机构：

[1] Shenyang Pharmaceut Univ, Sch Pharm, Shenyang, Peoples R China

[2] Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Minist Educ China, Yangzhou, Peoples R China

来源：

NATURAL PRODUCT RESEARCH | 2024年 / 38卷 / 14期

关键词：

Viburnum luzonicum Rolfe leaves; LC-MS analysis; chemical constituents; alpha-amylase inhibition; alpha-glucosidase inhibition; CONSTITUENTS;

D O I：

10.1080/14786419.2023.2173190

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Viburnum luzonicum Rolfe is widely used in China as folk medicine. The bioactivity evaluation indicated that the n-BuOH fraction of V. luzonicum leaves (VLLB) could significantly inhibit alpha-amylase and alpha-glucosidase. In order to clarify its active constituents, the phytochemical analysis on VLLB was first performed using HPLC-QTOF-MS/MS, and three new phenolic compounds, viburosides A-C (1-3), along with seven known analogues (4-10) were isolated through preparative HPLC. The undescribed compounds were determined by extensive spectroscopic analyses (H-1 and C-13 NMR, HSQC, HMBC, HRESIMS, and ORD) and enzymatic hydrolysis. In the in vitro enzyme assays, compounds 1-8 showed potent alpha-amylase and alpha-glucosidase inhibitory activities. The enzymatic kinetics and molecular docking of the strongest inhibitors 2 and 3 against the corresponding target enzyme were also performed.

引用

页码：2349 / 2356

页数：8