mScarlet3: a brilliant and fast-maturing red fluorescent protein

被引：34

作者：

Gadella, Theodorus W. J. ^{[1
,2
]}

van Weeren, Laura ^{[1
,2
]}

Stouthamer, Jente ^{[1
,2
]}

Hink, Mark A. ^{[1
,2
]}

Wolters, Anouk H. G. ^{[3
]}

Giepmans, Ben N. G. ^{[3
]}

Aumonier, Sylvain ^{[4
]}

Dupuy, Jerome ^{[4
]}

Royant, Antoine ^{[4
,5
]}

机构：

[1] Univ Amsterdam, Swammerdam Inst Life Sci, Sect Mol Cytol, Amsterdam, Netherlands

[2] Univ Amsterdam, Swammerdam Inst Life Sci, Van Leeuwenhoek Ctr Adv Microscopy, Amsterdam, Netherlands

[3] Univ Groningen UMC Groningen, Biomed Sci Cells & Syst, Groningen, Netherlands

[4] Univ Grenoble Alpes, CNRS, CEA, Inst Biol Structurale, Grenoble, France

[5] European Synchrotron Radiat Facil, Grenoble, France

来源：

NATURE METHODS | 2023年 / 20卷 / 04期

关键词：

MONOMERIC RED; FEATURES; ORANGE;

D O I：

10.1038/s41592-023-01809-y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the evolution of mScarlet3, a cysteine-free monomeric red fluorescent protein with fast and complete maturation, as well as record brightness, quantum yield (75%) and fluorescence lifetime (4.0 ns). The mScarlet3 crystal structure reveals a barrel rigidified at one of its heads by a large hydrophobic patch of internal residues. mScarlet3 behaves well as a fusion tag, displays no apparent cytotoxicity and it surpasses existing red fluorescent proteins as a Forster resonance energy transfer acceptor and as a reporter in transient expression systems. Two mScarlet variants with high brightness and fast maturation times have been evolved. These variants behave favorably as fusion tags and Forster resonance energy transfer acceptors.

引用

页码：541 / +

页数：27

共 30 条

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