Quantification of phospholipids and glycerides in human milk using ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry

被引：14

作者：

Liu, Yan ^{[1
,2
]}

Qiao, Weicang ^{[1
,2
]}

Liu, Yanpin ^{[1
,2
]}

Zhao, Junying ^{[1
,2
]}

Liu, Qian ^{[1
,2
]}

Yang, Kai ^{[1
,2
]}

Zhang, Minghui ^{[1
,2
]}

Wang, Yaling ^{[1
,2
]}

Chen, Lijun ^{[1
,2
]}

机构：

[1] Beijing Sanyuan Foods Co Ltd, Natl Engn Res Ctr Dairy Hlth Maternal & Child, Beijing, Peoples R China

[2] Beijing Sanyuan Foods Co Ltd, Beijing Engn Res Ctr Dairy, Beijing Tech Innovat Ctr Human Milk Res, Beijing, Peoples R China

来源：

FRONTIERS IN CHEMISTRY | 2023年 / 10卷

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

UPLC-Q-ToF-MS; human milk; lipids; phospholipids; glycerides; gangliosides; quantification; EVAPORATIVE LIGHT-SCATTERING; BOVINE-MILK; CURRENT KNOWLEDGE; INFANT FORMULAS; BREAST-MILK; LIPIDS; EXTRACTION; MEMBRANE; SAMPLES; PLASMA;

D O I：

10.3389/fchem.2022.1101557

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Human milk lipids, which are an important source of energy and affect growth and development of infants, require a comprehensive method for its qualitative and quantitative analysis. This work describes a method for the analysis of phospholipids, glycerides, free fatty acids and gangliosides in human milk by ultra-performance liquid chromatography using a C18 column with quadrupole-time-of-flight mass spectrometry (Q-TOF-MS). The lipids were extracted by liquid-liquid extraction and phospholipids were separated by solid phase extraction (SPE). The chromatographic columns with two different specifications (4.6 mm x 150 mm, and 3 mm x 50 mm) were used to detect phospholipids and glycerides in human milk, respectively. The sphingolipids and glycerides were analyzed in positive ion mode, and the glycerophospholipids and free fatty acids were analyzed in negative ion mode. Both internal and external standards were used for absolute quantification in this experiment. 483 species of lipids, including phospholipids, glycerides, free fatty acids and gangliosides, in human milk were analyzed using UPLC-Q-TOF-MS with high sensitivity and good linearity, with coefficient of correlation above 0.99, the relative standard deviation of accuracy and precision less than 10%. The results in a large number of human milk samples showed that this method was suitable for qualitative and quantitative analysis of lipids in human milk, even for other mammalian milk and infant formulae.

引用

页数：15

共 59 条

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