Establishment of an in situ model to explore the tumor immune microenvironment in head and neck squamous cell carcinoma

被引：1
作者：
Lu, Congxian [1 ,2 ,3 ]
Yang, Yuteng [2 ,3 ,4 ]
Zhang, Mingjun [1 ,2 ,3 ]
Li, Jiaxuan [1 ,2 ,3 ]
Song, Hao [2 ,3 ,4 ]
Zhao, Hongfei [1 ,2 ,3 ]
Mou, Yakui [1 ,2 ,3 ]
Li, Yumei [1 ,2 ,3 ,5 ]
Song, Xicheng [1 ,2 ,3 ,5 ]
机构：
[1] Qingdao Univ, Yantai Yuhuangding Hosp, Dept Otorhinolaryngol Head & Neck Surg, Qingdao, Shandong, Peoples R China
[2] Shandong Prov Clin Res Ctr Otorhinolaryngol Dis, Yantai, Shandong, Peoples R China
[3] Yantai Key Lab Otorhinolaryngol Dis, Yantai, Shandong, Peoples R China
[4] Binzhou Med Univ, Med Coll 2, Yantai, Shandong, Peoples R China
[5] Qingdao Univ, Yantai Yuhuangding Hosp, Dept Otolaryngol Head & Neck Surg, Yantai 264000, Peoples R China
来源：
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK | 2024年 / 46卷 / 06期
关键词：
head and neck squamous cell carcinoma; immunohistochemistry; lymph node metastasis; tumor growth; tumor microenvironment; tumorigenesis; MURINE MODEL; MOUSE MODEL; CANCER; EPIDEMIOLOGY; DIAGNOSIS; PD-L1; MICE;
D O I：
10.1002/hed.27707
中图分类号：
R76 [耳鼻咽喉科学];
学科分类号：
100213 ;
摘要：
ObjectiveEstablish an in situ model for investigating HNSCC, focusing on tumor growth, metastasis, and the immune microenvironment.MethodsGenerated a monoclonal SCCVII-ZsGreen cell line through lentiviral transfection. Selected monoclonal lines with growth rates similar to the original SCCVII for in vivo tumorigenesis. Monitored tumor development and metastasis through fluorescence in vivo imaging. Employed immunohistochemistry to assess immune cell distribution in the tumor microenvironment.ResultsSCCVII-ZsGreen exhibited comparable proliferation and in vivo tumorigenicity to SCCVII. In situ tumor formation on day 10, with cervical metastasis in C57BL/6 mice by day 16. No significant fluorescence signals in organs like liver and lungs, while SCCVII-ZsGreen presence confirmed in cervical lymph node metastases. Immunohistochemistry revealed CD4+ T, CD8+ T, B, and dendritic cells distribution, with minimal macrophages.ConclusionOur model is a valuable tool for studying HNSCC occurrence, metastasis, and immune microenvironment. It allows dynamic observation of tumor development, aids preclinical drug experiments, and facilitates exploration of the tumor immune contexture.
引用
收藏
页码：1310 / 1321
页数：12
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