Establishment of an in situ model to explore the tumor immune microenvironment in head and neck squamous cell carcinoma

被引：1
作者：
Lu, Congxian [1 ,2 ,3 ]
Yang, Yuteng [2 ,3 ,4 ]
Zhang, Mingjun [1 ,2 ,3 ]
Li, Jiaxuan [1 ,2 ,3 ]
Song, Hao [2 ,3 ,4 ]
Zhao, Hongfei [1 ,2 ,3 ]
Mou, Yakui [1 ,2 ,3 ]
Li, Yumei [1 ,2 ,3 ,5 ]
Song, Xicheng [1 ,2 ,3 ,5 ]
机构：
[1] Qingdao Univ, Yantai Yuhuangding Hosp, Dept Otorhinolaryngol Head & Neck Surg, Qingdao, Shandong, Peoples R China
[2] Shandong Prov Clin Res Ctr Otorhinolaryngol Dis, Yantai, Shandong, Peoples R China
[3] Yantai Key Lab Otorhinolaryngol Dis, Yantai, Shandong, Peoples R China
[4] Binzhou Med Univ, Med Coll 2, Yantai, Shandong, Peoples R China
[5] Qingdao Univ, Yantai Yuhuangding Hosp, Dept Otolaryngol Head & Neck Surg, Yantai 264000, Peoples R China
来源：
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK | 2024年 / 46卷 / 06期
关键词：
head and neck squamous cell carcinoma; immunohistochemistry; lymph node metastasis; tumor growth; tumor microenvironment; tumorigenesis; MURINE MODEL; MOUSE MODEL; CANCER; EPIDEMIOLOGY; DIAGNOSIS; PD-L1; MICE;
D O I：
10.1002/hed.27707
中图分类号：
R76 [耳鼻咽喉科学];
学科分类号：
100213 ;
摘要：
ObjectiveEstablish an in situ model for investigating HNSCC, focusing on tumor growth, metastasis, and the immune microenvironment.MethodsGenerated a monoclonal SCCVII-ZsGreen cell line through lentiviral transfection. Selected monoclonal lines with growth rates similar to the original SCCVII for in vivo tumorigenesis. Monitored tumor development and metastasis through fluorescence in vivo imaging. Employed immunohistochemistry to assess immune cell distribution in the tumor microenvironment.ResultsSCCVII-ZsGreen exhibited comparable proliferation and in vivo tumorigenicity to SCCVII. In situ tumor formation on day 10, with cervical metastasis in C57BL/6 mice by day 16. No significant fluorescence signals in organs like liver and lungs, while SCCVII-ZsGreen presence confirmed in cervical lymph node metastases. Immunohistochemistry revealed CD4+ T, CD8+ T, B, and dendritic cells distribution, with minimal macrophages.ConclusionOur model is a valuable tool for studying HNSCC occurrence, metastasis, and immune microenvironment. It allows dynamic observation of tumor development, aids preclinical drug experiments, and facilitates exploration of the tumor immune contexture.
引用
收藏
页码：1310 / 1321
页数：12
相关论文
共 46 条
[1]  
Brand M., 2020, INT J MOL SCI, V22:22010247
[2]   Tumor immune microenvironment in head and neck cancers [J].
Chen, Samantha M. Y. ;
Krinsky, Alexandra L. ;
Woolaver, Rachel A. ;
Wang, Xiaoguang ;
Chen, Zhangguo ;
Wang, Jing H. .
MOLECULAR CARCINOGENESIS, 2020, 59 (07) :766-774
[3]   DEVELOPMENT OF A NEW INVIVO MODEL FOR HEAD AND NECK-CANCER [J].
DINESMAN, A ;
HAUGHEY, B ;
GATES, GA ;
AUFDEMORTE, T ;
VONHOFF, DD .
OTOLARYNGOLOGY-HEAD AND NECK SURGERY, 1990, 103 (05) :766-774
[4]   Tumor microenvironment and immune evasion in head and neck squamous cell carcinoma [J].
Elmusrati, Areeg ;
Wang, Justin ;
Wang, Cun-Yu .
INTERNATIONAL JOURNAL OF ORAL SCIENCE, 2021, 13 (01)
[5]   An HNSCC syngeneic mouse model for tumor immunology research and preclinical evaluation [J].
Fu, You ;
Tian, Guocai ;
Li, Jiang ;
Zhang, Zhiyuan ;
Xu, Ke .
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2020, 46 (04) :1501-1513
[6]   The prognostic influence of tumour-infiltrating lymphocytes in cancer: a systematic review with meta-analysis [J].
Gooden, M. J. M. ;
de Bock, G. H. ;
Leffers, N. ;
Daemen, T. ;
Nijman, H. W. .
BRITISH JOURNAL OF CANCER, 2011, 105 (01) :93-103
[7]   Targeting Hypoxia-Inducible Factor-1α/Pyruvate Dehydrogenase Kinase 1 Axis by Dichloroacetate Suppresses Bleomycin-induced Pulmonary Fibrosis [J].
Goodwin, Justin ;
Choi, Hyunsung ;
Hsieh, Meng-hsiung ;
Neugent, Michael L. ;
Ahn, Jung-Mo ;
Hayenga, Heather N. ;
Singh, Pankaj K. ;
Shackelford, David B. ;
Lee, In-Kyu ;
Shulaev, Vladimir ;
Dhar, Shanta ;
Takeda, Norihiko ;
Kim, Jung-whan .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2018, 58 (02) :216-231
[8]   Effector CD4 and CD8 T Cells and Their Role in the Tumor Microenvironment [J].
Hadrup, Sine ;
Donia, Marco ;
Straten, Per thor .
CANCER MICROENVIRONMENT, 2013, 6 (02) :123-133
[9]   Functional in vivo optical imaging of tumor angiogenesis, growth, and metastasis prevented by administration of anti-human VEGF antibody in xenograft model of human fibrosarcoma HT1080 cells [J].
Hanyu, Aki ;
Kojima, Kiyotsugu ;
Hatake, Kiyohiko ;
Nomura, Kimie ;
Murayama, Hironori ;
Ishikawa, Yuichi ;
Miyata, Satoshi ;
Ushijima, Masaru ;
Matsuura, Masaaki ;
Ogata, Etsuro ;
Miyazawa, Keiji ;
Imamura, Takeshi .
CANCER SCIENCE, 2009, 100 (11) :2085-2092
[10]   In vivo optical imaging of cancer cell function and tumor microenvironment [J].
Imamura, Takeshi ;
Saitou, Takashi ;
Kawakami, Ryosuke .
CANCER SCIENCE, 2018, 109 (04) :912-918
12345