Microcystin-leucine arginine promotes colorectal cancer cell proliferation by activating the PI3K/Akt/Wnt/β-catenin pathway

被引：11
作者：
Tang, Yaqi [1 ]
Yi, Xiaoyu [1 ]
Zhang, Xinyu [2 ]
Liu, Baojie [1 ]
Lu, Yongzheng [1 ]
Pan, Zhifang [1 ]
Yu, Tao [1 ]
Feng, Weiguo [1 ]
机构：
[1] Weifang Med Univ, Sch Life Sci & Technol, Weifang 261053, Shandong, Peoples R China
[2] Shandong First Med Univ, Dept Gastrointestinal Surg, Affiliated Hosp 2, Tai An 271000, Shandong, Peoples R China
来源：
ONCOLOGY REPORTS | 2023年 / 49卷 / 01期
基金：
中国国家自然科学基金;
关键词：
microcystin-leucine arginine; colorectal cancer; proliferation; PI3K; Akt; Wnt; beta-catenin; LR EXPOSURE; APOPTOSIS; MIGRATION; LAKE;
D O I：
10.3892/or.2022.8455
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Microcystin-leucine arginine (MC-LR) is an environmental toxin produced by cyanobacteria and is considered to be a potent carcinogen. However, to the best of our knowledge, the effect of MC-LR on colorectal cancer (CRC) cell proliferation has never been studied. The aim of the present study was to investigate the effect of MC-LR on CRC cell proliferation and the underlying mechanisms. Firstly, a Cell Counting Kit-8 (CCK-8) assay was conducted to determine cell viability at different concentrations, and 50 nM MC-LR was chosen for further study. Subsequently, a longer CCK-8 assay and a cell colony formation assay showed that MC-LR promoted SW620 and HT29 cell proliferation. Furthermore, western blotting analysis showed that MC-LR significantly upregulated protein expression of PI3K, p-Akt (Ser473), p-GSK3 beta (Ser9), beta-catenin, c-myc and cyclin D1, suggesting that MC-LR activated the PI3K/Akt and Wnt/beta-catenin pathways in SW620 and HT29 cells. Finally, the pathway inhibitors LY294002 and ICG001 were used to validate the role of the PI3K/Akt and Wnt/beta-catenin pathways in MC-LR-accelerated cell proliferation. The results revealed that MC-LR activated Wnt/beta-catenin through the PI3K/Akt pathway to promote cell proliferation. Taken together, these data showed that MC-LR promoted CRC cell proliferation by activating the PI3K/Akt/Wnt/beta-catenin pathway. The present study provided a novel insight into the toxicological mechanism of MC-LR.
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页数：8
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