Chemical Synthesis of Peptides and Proteins Bearing Base-Labile Post-Translational Modifications: Evolution of the Methods in Four Decades

被引：6
作者：
Ma, Wenjie [1 ]
Liu, Han [1 ]
Li, Xuechen [1 ]
机构：
[1] Univ Hong Kong, Dept Chem, State Key Lab Synthet Chem, Pokfulam Rd, Hong Kong, Peoples R China
来源：
CHEMBIOCHEM | 2023年 / 24卷 / 20期
关键词：
base-labile posttranslation modification; chemical protein synthesis; chemical ligation; S-palmitoylation; O-acetylation; SOLID-PHASE SYNTHESIS; MYELIN PROTEOLIPID PROTEIN; LIPID-MODIFIED PROTEINS; SAFETY-CATCH LINKER; N-RAS PROTEIN; CHEMOENZYMATIC SYNTHESIS; CHARACTERISTIC LIPOPEPTIDES; TERMINAL LIPOHEXAPEPTIDE; EFFICIENT SYNTHESIS; ENZYMATIC-SYNTHESIS;
D O I：
10.1002/cbic.202300348
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The S-palmitoylation on Cys residue and O-acetylation on Ser/Thr residues are two types of base-labile post-translational modifications (PTMs) in cells. The lability of these PTMs to bases and nucleophiles makes the peptides/proteins bearing S-palmitoyl or O-acetyl groups challenging synthetic targets, which cannot be prepared via the standard Fmoc-SPPS and native chemical ligation. In this review, we summarized the efforts towards their preparation in the past 40 years, with the focus on the evolution of synthetic methods.
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页数：12
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