Blood parasite load by qPCR as therapeutic monitoring in visceral leishmaniasis patients in Brazil: a case series study

被引：3

作者：

de Aquino, Samuel Ricarte ^{[1
,2
]}

Diniz, Lucyo Flavio Bezerra ^{[1
,2
]}

Nunes, Savio Luiz Pereira ^{[3
]}

Silva, Roberta Lane de Oliveira ^{[4
]}

Gouveia, Gisele Veneroni ^{[5
]}

Gouveia, Joao Jose Simoni ^{[5
]}

Sales, Kamila Gaudencio da Silva ^{[6
]}

Dantas-Torres, Filipe ^{[6
]}

do Carmo, Rodrigo Feliciano ^{[1
,3
]}

机构：

[1] Univ Fed Vale Sao Francisco, Programa Posgrad Ciencias Saude & Biol, Petrolina, PE, Brazil

[2] Univ Fed Vale Sao Francisco, Hosp Univ, Petrolina, PE, Brazil

[3] Univ Pernambuco, Programa Posgrad Biol Celular & Mol Aplicada, Recife, PE, Brazil

[4] Univ Fed Vale Sao Francisco, Lab Microbiol Geral, Petrolina, PE, Brazil

[5] Univ Fed Vale Sao Francisco, Colegiado Zootecnia, Petrolina, PE, Brazil

[6] Inst Aggeu Magalhaes, Dept Imunol, Recife, PE, Brazil

来源：

REVISTA DA SOCIEDADE BRASILEIRA DE MEDICINA TROPICAL | 2023年 / 56卷

关键词：

Kala-azar; Leishmania infantum; qPCR; Relapse; Visceral leishmaniasis; TIME PCR ASSAY; KINETOPLAST DNA; INFANTUM; QUANTIFICATION;

D O I：

10.1590/0037-8682-0456-2022

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Background: This study aimed to describe the kinetics of Leishmania parasite load determined using kinetoplast DNA (kDNA)-based quantitative polymerase chain reaction (qPCR) in visceral leishmaniasis (VL) patients. Methods: Parasite load in blood was assessed by qPCR at five time points, up to 12 months post-diagnosis. Sixteen patients were followed up. Results: A significant reduction in the parasite load was observed after treatment (P < 0.0001). One patient had an increased parasite load 3 months post-treatment and relapsed clinically at month six. Conclusions: We have described the use of kDNA-based qPCR in the post-treatment follow-up of VL cases.

引用

页数：7

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