ChrR Gene Variability in Cr-stressed Leptolyngbya boryana for the Biotransformation of Cr (VI) to Cr (III)

被引：1
|
作者：
Yadav, Ajit Pratap Singh [1 ]
Dwivedi, Vinay [2 ]
Kumar, Satyendra [3 ]
Kumar, Anil [4 ]
机构：
[1] Rama Inst Engn & Technol, Dept Biotechnol, Kanpur, Uttar Pradesh, India
[2] Chandigarh Univ, Dept Biotechnol Engn & Food Technol, Chandigarh, India
[3] BNM Univ, BNMV Coll, Dept Bot, Madhepura, Bihar, India
[4] Rama Med Coll Hosp & Res Ctr, Cent Res Lab, Kanpur, Uttar Pradesh, India
来源：
JOURNAL OF PURE AND APPLIED MICROBIOLOGY | 2023年 / 17卷 / 01期
关键词：
Bioremediation; ChrR gene; Leptolyngbya Boryana; Genetic Variability; Chromium Reductase; Homology; HEXAVALENT CHROMIUM; REDUCTION; BACTERIA; IDENTIFICATION; TOXICITY;
D O I：
10.22207/JPAM.17.1.36
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Chromium (VI) is a well-known pollutant that is present in industrially polluted soil and water, and has been reported to be mutagenic and carcinogenic. In the present study, we investigated the effective use of Leptolyngbya boryana (cyanobacterium) as an eco-friendly option to overcome Cr (VI) toxicity in tannery effluents. The main objective of this study was to identify the Cr reductase (ChrR) gene and its variability in the context of Cr (VI) stress. Industrial polluted soil samples were collected and processed according to standard protocols for ChrR variation and 16S rDNA gene analysis. Genomic DNA was isolated from the collected samples and the ChrR and 16S rDNA genes were amplified by PCR. Amplified 16S rDNA was sequenced and aligned with known sequences. In the present study, a strong correlation was established between the nucleotide sequences of the ChrR and 16S rDNA genes. The Minimum Inhibitory Concentration (MIC) was determined for Cr (VI), and pure strains of L. boryana were identified and isolated from soil samples. Cr (VI)-stressed conditions and their genetic variability were confirmed by sequencing. In conclusion, the L. boryana strain has been identified an eco-friendly option for overcoming Cr (VI) toxicity in tannery effluents.
引用
收藏
页码：439 / 448
页数：10
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