Zeolitic imidazolate frameworks activate endosomal Toll-like receptors and potentiate immunogenicity of SARS-CoV-2 spike protein trimer

被引：9

作者：

Alsaiari, Shahad K. ^{[1
]}

Nadeef, Seba ^{[1
]}

Daristotle, John L. ^{[1
]}

Rothwell, William ^{[1
,2
]}

Du, Bujie ^{[1
]}

Garcia, Johnny ^{[1
]}

Zhang, Linzixuan ^{[3
]}

Sarmadi, Morteza ^{[1
]}

Forster, Timothy A. ^{[1
]}

Menon, Nandita ^{[1
]}

Lin, Stacey Qiaohui ^{[1
]}

Tostanoski, Lisa H. ^{[4
]}

Hachmann, Nicole ^{[4
]}

Wang, Erika Yan ^{[1
]}

Ventura, John D. ^{[4
]}

Barouch, Dan H. ^{[4
]}

Langer, Robert ^{[1
,3
]}

Jaklenec, Ana ^{[1
]}

机构：

[1] MIT, David H Koch Inst Integrat Canc Res, Cambridge, MA 02139 USA

[2] Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA

[3] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

[4] Harvard Med Sch, Beth Israel Deaconess Med Ctr, Ctr Virol & Vaccine Res, Boston, MA 02215 USA

来源：

SCIENCE ADVANCES | 2024年 / 10卷 / 10期

关键词：

PATHOGEN RECOGNITION; SUBUNIT VACCINE; RESPONSES; AGONISTS; IMMUNE; INFECTION; ROLES;

D O I：

10.1126/sciadv.adj6380

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nanomaterials offer unique opportunities to engineer immunomodulatory activity. In this work, we report the Toll-like receptor agonist activity of a nanoscale adjuvant zeolitic imidazolate framework-8 (ZIF-8). The accumulation of ZIF-8 in endosomes and the pH-responsive release of its subunits enable selective engagement with endosomal Toll-like receptors, minimizing the risk of off-target activation. The intrinsic adjuvant properties of ZIF-8, along with the efficient delivery and biomimetic presentation of a severe acute respiratory syndrome coronavirus 2 spike protein receptor-binding domain trimer, primed rapid humoral and cell-mediated immunity in a dose-sparing manner. Our study offers insights for next-generation adjuvants that can potentially impact future vaccine development.

引用

页数：11

共 69 条

[1] Evaluation of Efficacy, Biodistribution, and Inflammation for a Potent siRNA Nanoparticle: Effect of Dexamethasone Co-treatment
Abrams, Marc T.
Koser, Martin L.
Seitzer, Jessica
Williams, Stephanie C.
DiPietro, Martha A.
Wang, Weimin
Shaw, Andrew W.
Mao, Xianzhi
Jadhav, Vasant
Davide, Joseph P.
Burke, Paul A.
Sachs, Alan B.
Stirdivant, Steven M.
Sepp-Lorenzino, Laura
[J]. MOLECULAR THERAPY, 2010, 18 (01) : 171 - 180
[2] Pathogen recognition and innate immunity
Akira, S
Uematsu, S
Takeuchi, O
[J]. CELL, 2006, 124 (04) : 783 - 801
[3] Endosomal Escape and Delivery of CRISPR/Cas9 Genome Editing Machinery Enabled by Nanoscale Zeolitic Imidazolate Framework
Alsaiari, Shahad K.
Patil, Sachin
Alyami, Mram
Alamoudi, Kholod O.
Aleisa, Fajr A.
Merzaban, Jasmeen S.
Li, Mo
Khashab, Niveen M.
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2018, 140 (01) : 143 - 146
[4] Pattern Recognition Receptors and the Host Cell Death Molecular Machinery
Amarante-Mendes, Gustavo P.
Adjemian, Sandy
Branco, Laura Migliari
Zanetti, Larissa C.
Weinlich, Ricardo
Bortoluci, Karina R.
[J]. FRONTIERS IN IMMUNOLOGY, 2018, 9
[5] Differential expression analysis for sequence count data
Anders, Simon
Huber, Wolfgang
[J]. GENOME BIOLOGY, 2010, 11 (10):
[6] Baxan N., 2019, Dis. Model. Mech, V12, pdmm040725
[7] Identification of a Human Toll-Like Receptor (TLR) 8-Specific Agonist and a Functional Pan-TLR Inhibitor in 2-Aminoimidazoles
Beesu, Mallesh
Caruso, Giuseppe
Salyer, Alex C. D.
Shukla, Nijunj M.
Khetani, Karishma K.
Smith, Luke J.
Fox, Lauren M.
Tanji, Hiromi
Ohto, Umeharu
Shimizu, Toshiyuki
David, Sunil A.
[J]. JOURNAL OF MEDICINAL CHEMISTRY, 2016, 59 (07) : 3311 - 3330
[8] Robust IgG responses to nanograms of antigen using a biomimetic lipid-coated particle vaccine
Bershteyn, Anna
Hanson, Melissa C.
Crespo, Monica P.
Moon, James J.
Li, Adrienne V.
Suh, Heikyung
Irvine, Darrell J.
[J]. JOURNAL OF CONTROLLED RELEASE, 2012, 157 (03) : 354 - 365
[9] Evolution of Toll-like receptor 7/8 agonist therapeutics and their delivery approaches: From antiviral formulations to vaccine adjuvants
Bhagchandani, Sachin
Johnson, Jeremiah A.
Irvine, Darrell J.
[J]. ADVANCED DRUG DELIVERY REVIEWS, 2021, 175
[10] INFLUENZA VIRUS-INFECTED DENDRITIC CELLS STIMULATE STRONG PROLIFERATIVE AND CYTOLYTIC RESPONSES FROM HUMAN CD8+ T-CELLS
BHARDWAJ, N
BENDER, A
GONZALEZ, N
BUI, LK
GARRETT, MC
STEINMAN, RM
[J]. JOURNAL OF CLINICAL INVESTIGATION, 1994, 94 (02) : 797 - 807

← 1 2 3 4 5 6 7 →