In vitro mucin degradation and paracellular permeability by fecal water from Crohn's disease patients

被引：1
作者：
Becker, Heike E. F. [1 ,2 ]
Kameli, Nader [2 ,3 ]
Rustichelli, Alice
Heijnens, Britt A. M. [1 ]
Stassen, Frank R. M. [2 ]
Penders, John [2 ,4 ]
Jonkers, Daisy M. A. E. [1 ]
机构：
[1] Maastricht Univ, Med Ctr, NUTRIM Sch Nutr & Translat Res Metab, Dept Gastroenterol Hepatol,Div Internal Med, Maastricht, Netherlands
[2] Maastricht Univ, Med Ctr, NUTRIM Sch Nutr & Translat Res Metab, Dept Med Microbiol, Maastricht, Netherlands
[3] Jazan Univ, Fac Appl Med Sci, Dept Med Microbiol, Jazan, Saudi Arabia
[4] Maastricht Univ, Med Ctr, Caphri Sch Publ Hlth & Primary Care, Dept Med Microbiol, Maastricht, Netherlands
来源：
FUTURE MICROBIOLOGY | 2024年 / 19卷 / 04期
基金：
欧盟地平线“2020”;
关键词：
Crohn's disease; fecal microbial secretome; intestinal epithelial barrier; mucin; INFLAMMATORY-BOWEL-DISEASE; TIGHT JUNCTIONS; BARRIER; BACTERIA; MUCUS; SEQUENCES; HEALTH;
D O I：
10.2217/fmb-2022-0265
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: This study aimed to examine the impact of fecal water (FW) of active and remissive Crohn's disease (CD) patients on mucin degradation and epithelial barrier function. Methods: FW and bacterial membrane vesicles (MVs) were isolated from fresh fecal samples of six healthy controls (HCs) and 12 CD patients. Bacterial composition was determined by 16S rRNA gene amplicon sequencing. Results: In vitro FW-induced mucin degradation was higher in CD samples versus HC (p < 0.01), but not associated with specific bacterial genera. FW of three remissive samples decreased transepithelial electrical resistance in Caco-2 cells by 78-87% (p < 0.001). MVs did not induce barrier alterations. Conclusion: The higher mucin-degradation capacity of CD-derived FW might suggest contributions of microbial products to CD pathophysiology.
引用
收藏
页码：335 / 347
页数：13
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