White blood cell subtypes and neutrophil extracellular traps content as biomarkers for stroke etiology in acute ischemic stroke clots retrieved by mechanical thrombectomy

被引：9

作者：

Jabrah, Duaa ^{[1
]}

Rossi, Rosanna ^{[1
,2
]}

Molina, Sara ^{[1
,2
]}

Douglas, Andrew ^{[1
]}

Pandit, Abhay ^{[1
,2
]}

McCarthy, Ray ^{[3
]}

Gilvarry, Michael ^{[3
]}

Ceder, Eric ^{[4
]}

Fitzgerald, Sean ^{[1
]}

Dunker, Dennis ^{[4
]}

Nordanstig, Annika ^{[5
,6
]}

Redfors, Petra ^{[5
,6
]}

Tatlisumak, Turgut ^{[5
,6
]}

O'Hare, Alan ^{[7
]}

Power, Sarah ^{[7
]}

Brennan, Paul ^{[7
]}

Owens, Peter ^{[11
]}

Nagy, Andras ^{[7
,8
]}

Vadasz, Agnes ^{[6
,7
]}

De Meyer, Simon F. ^{[8
]}

Tsivgoulis, Georgios ^{[9
]}

Psychogios, Klearchos ^{[10
]}

Szikora, Istvan ^{[8
]}

Jood, Katarina ^{[5
,6
]}

Rentzos, Alexandros ^{[4
]}

Thornton, John ^{[7
]}

Doyle, Karen ^{[1
,2
,12
]}

机构：

[1] Univ Galway, Dept Physiol, Galway, Ireland

[2] Univ Galway, CURAM SFI Res Ctr Med Devices, Galway, Ireland

[3] Galway Neuro Technol Ctr, Cerenovus, Galway, Ireland

[4] Dept Intervent Diagnost Neuroradiol, Dept Intervent & Diagnost Neuroradiol, Inst Clin Sci, Sahlgrenska Acad,Sahlgrenska Univ Hosp,Dept Radi, Gothenburg, Sweden

[5] Univ Gothenburg, Inst Neurosci & Physiol, Sahlgrenska Acad, Dept Clin Neurosci, Gothenburg, Sweden

[6] Sahlgrens Univ Hosp, Dept Neurol, Gothenburg, Sweden

[7] Beaumont Hosp, Royal Coll Surg Ireland, Dept Radiol, Dublin, Ireland

[8] Natl Inst Neurosci, Dept Neurointervent, Budapest, Hungary

[9] Natl & Kapodistrian Univ Athens, Attikon Univ Hosp, Dept Neurol 2, Athens, Greece

[10] Metropolitan Hosp, Stroke Unit, Piraeus, Greece

[11] Univ Galway, Ctr Microscopy & Imaging, Galway, Ireland

[12] Univ Galway, Dept Physiol, Galway H91TK33, Ireland

来源：

THROMBOSIS RESEARCH | 2024年 / 234卷

基金：

爱尔兰科学基金会;

关键词：

Ischemic stroke; Etiology; White blood cells; Neutrophils; Lymphocytes; Macrophages; Neutrophil extracellular traps; THROMBUS; EXPRESSION; RATIO;

D O I：

10.1016/j.thromres.2023.12.005

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background: Lymphocytes, macrophages, neutrophils, and neutrophil extracellular traps (NETs) associate with stroke risk factors and form a thrombus through different mechanisms. We investigated the total WBCs, WBC subtypes and NETs composition in acute ischemic stroke (AIS) clots to identify possible etiological differences that could help us further understand the process of thrombosis that leads to AIS.Methods: AIS clots from 100 cases each of atherothrombotic (AT), cardioembolic (CE) and cryptogenic stroke etiology were collected per-pass as part of the CuRAM RESTORE registry of AIS clots. Martius Scarlet Blue stain was used to identify the main histological components of the clots. Immunohistochemical staining was used to identify neutrophils, lymphocytes, macrophages, and NETs patterns. The cellular and histological components were quantified using Orbit Image Analysis software.Results: AT clots were larger, with more red blood cells and fewer WBCs than CE clots. AT clots had more lymphocytes and cryptogenic clots had fewer macrophages than other etiologies. Most significantly, CE clots showed higher expression of neutrophils and extracellular web-like NETs compared to AT and cryptogenic clots. There was also a significantly higher distribution of web-like NETs around the periphery of the CE clots while a mixed distribution was observed in AT clots.Conclusion: The difference in neutrophil and NETs expression in clots from different etiologies may provide insight into the mechanism of clot formation.

引用

页码：1 / 8

页数：8