IN VITRO CULTIVATION OF SAFFRON (CROCUS SATIVUS L.) AND ASSESSMENT OF GENETIC HOMOGENEITY USING IPBS MARKERS

被引：0
作者：
Petrova, Maria [1 ]
Bonchev, Georgi [1 ]
Dimitrova, Lyudmila [1 ]
Dimitrova, Margarita [1 ]
Vassilevska-Ivanova, Roumiana [1 ]
机构：
[1] Bulgarian Acad Sci, Inst Plant Physiol & Genet, Acad G Bonchev Str Bl 21, Sofia 1113, Bulgaria
来源：
COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES | 2023年 / 76卷 / 11期
关键词：
corm; tissue culture; organogenesis; surface sterilization; molecular markers; SILVER-NITRATE; MICROPROPAGATION; EXPLANTS; CORMS;
D O I：
10.7546/CRABS.2023.11.04
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
This research was an attempt to develop a protocol for the most optimal in vitro cultivation of saffron (Crocus sativus L.). The dried stigmas of the plant are known as spice imparting colour, flavour and aroma to foods and beverages. The appropriate disinfection procedure to introduce corm explants into in vitro culture was established. The optimum corm formation and corm growth (mean number of 3.6 per explant) was achieved on Murashige and Skoog (MS) medium containing 5 mg/l 6-benzylaminopurine (BAP) and 1 mg/l indole-3-acetic acid (IAA), while MS supplemented with 1 mg/l indole-3-butyric acid (IBA) was the best medium for root development. The fingerprinting profiles of the field saffron and the in vitro cultures assessed by iPBS markers were found identical which implies that no genetic alterations have occurred in response to in vitro cultivation.
引用
收藏
页码：1670 / 1678
页数：9
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