Total phenolic and flavonoid contents and antioxidant, antimicrobial and antiproliferative activities of Polycarpon tetraphyllum

Plants are organic materials that support a variety of biological processes. The biological activity of the Polycarpon tetraphyllum (L.) L plant was identified in this study. In this case, the Soxhlet apparatus was used to obtain ethanol and methanol extracts of the plant's aerial portions. Total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) values for the plant were determined using Rel Assay kits. The antioxidant potential of the plant extract was determined by the DPPH test. Antimicrobial activity was determined using the agar dilution test against six bacterial and three fungal strains. The antiproliferative activity of the plant extract against A549 lung cancer cell line was determined by MTT assay. Phenolic compounds in the plant were screened with LC-MS/ MS device. As a result of the study, the DPPH activity of the plant was found to be higher in the methanol extract (75.72 & PLUSMN; 0.32) at a concentration of 2 mg/mL. TAS value of the plant was determined as 8.449 & PLUSMN; 0.143 mmol/L, TOS value as 12.129 & PLUSMN; 0.200 & mu;mol/L and OSI value as 0.144 & PLUSMN; 0.004. It was determined that the plant extracts showed the highest effect against Acinetobacter baumannii, Candida albicans and C. krusei at 50 & mu;g/mL. The ethanol extract of the plant was found to have stronger antiproliferative activity. In addition, the presence of acetohydroxamic acid, catechin hydrate, resveratrol, fumaric acid, 4-hydroxycinnamic acid, 4-gydroxybenzoic acid, oleuropein, ellagic acid, quercetin, butein and luteolin were found in the plant. In this context, it was determined that the plant has important biological activity.