In Vitro Expression Analysis of Cytokines and ROS-Related Genes in Human Corneal Fibroblasts and Keratocytes of Healthy and Keratoconus Corneas

被引：7

作者：

Berger, Tim ^{[1
]}

Szentmary, Nora ^{[2
]}

Chai, Ning ^{[2
]}

Flockerzi, Elias ^{[1
]}

Daas, Loay ^{[1
]}

Stachon, Tanja ^{[2
]}

Seitz, Berthold ^{[1
]}

机构：

[1] Saarland Univ, Med Ctr, Dept Ophthalmol, Homburg, Germany

[2] Saarland Univ, Dr Rolf M Schwiete Ctr Limbal Stem Cell & Congen, Homburg, Germany

来源：

OCULAR IMMUNOLOGY AND INFLAMMATION | 2024年 / 32卷 / 05期

关键词：

Corneal fibroblasts; cytokines; inflammation; keratoconus; keratocytes; reactive oxygen species; TRANSFORMING-GROWTH-FACTOR; INFLAMMATORY MOLECULES; APOPTOSIS; TEARS; STRESS;

D O I：

10.1080/09273948.2023.2176325

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

PurposeTo investigate expression of cytokines and ROS-related genes in stromal cells of healthy and keratoconus (KC) corneas.MethodsExpression analysis was performed for cytokines including several interleukins (IL), Tumor necrosis factor-alpha (TNF-alpha), Transforming growth factor-beta 1 (TGF-beta 1), Interferon-gamma (IFN-gamma) and ROS-related genes such as Catalase, Glutathione peroxidase 1, NADPH oxidase 1, superoxide dismutase 1 in corneal fibroblasts (HCFs/KC-HCFs) or keratocytes (Keratocytes/KC-Keratocytes) by qPCR and ELISA.ResultsGene and protein expression of most inflammatory markers was decreased in keratocytes compared to fibroblasts, whereas no differences were found between healthy and keratoconus cells for the majority of cytokines measured. TNF-alpha expression was increased at gene (KC keratocytes) and protein levels (supernatant of Keratocytes/KC-Keratocytes) compared to corneal fibroblasts. No differential expression of ROS-related genes was detected between healthy and diseased cells in both fibroblasts and keratocytes.ConclusionIncreased expression of several inflammatory markers described as altered in KC was not evident in KC cells in vitro.

引用

页码：556 / 565

页数：10

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