LC-MS/MS method for simultaneous estimation of raloxifene, cladrin in rat plasma: application in pharmacokinetic studies

被引：4

作者：

Chauhan, Divya ^{[1
,2
]}

Dadge, Shailesh ^{[1
,2
]}

Yadav, Pavan K. ^{[1
,2
]}

Sultana, Nazneen ^{[1
]}

Agarwal, Arun ^{[1
,2
]}

Vishwakarma, Sachin ^{[1
]}

Rathaur, Shivam ^{[1
]}

Yadav, Shubhi ^{[1
]}

K Chourasia, Manish ^{[1
,2
]}

Gayen, Jiaur R. ^{[1
,2
]}

机构：

[1] Cent Drug Res Inst, Div Pharmaceut & Pharmacokinet, Lucknow 226031, Uttar Pradesh, India

[2] Acad Sci & Innovat Res, Ghaziabad 201002, India

来源：

BIOANALYSIS | 2024年 / 16卷 / 03期

关键词：

bioanalytical method validation; co-estimation; LC-MS/MS; pharmacokinetics; raloxifene and cladrin; simultaneous identification; ESTROGEN-RECEPTOR MODULATORS; UPLC-MS/MS; VALIDATION; OSTEOPOROSIS; METABOLITES; ASSAY;

D O I：

10.4155/bio-2023-0206

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aim: A newer LC-MS/MS method was developed and validated for the simultaneous quantification of raloxifene (RL) and cladrin (CL). Methodology: Both drugs were resolved in RP-18 (4.6 x 50 mm, 5 mu) Xbridge Shield column using acetonitrile and 0.1% aqueous solution of formic acid (FA) (70:30% v/v) as mobile phase by using biological matrices in female Sprague-Dawley rats using-MS/MS. Results: The developed method was found to be linear over the concentration ranges of 1-600 ng/ml, and lower limit of quantification was 1 ng/ml for RL and CL, respectively. Pharmacokinetic results of RL+CL showed C-max = 4.23 +/- 0.61, 26.97 +/- 1.14 ng/ml, at T-max(h) 5.5 +/- 1.00 and 3.5 +/- 1.00, respectively. Conclusion: Pharmacokinetic study results will be useful in the future for the combined delivery of RL and CL for osteoporosis treatment. [GRAPHICS] .

引用

页码：141 / 153

页数：14

共 42 条

[41] Enhanced apoptosis and mitochondrial cell death by paclitaxel-loaded TPP-TPGS1000-functionalized nanoemulsion
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[J]. NANOMEDICINE, 2023, 18 (04) : 343 - 366
[42] HPLC method for simultaneous estimation of paclitaxel and baicalein: pharmaceutical and pharmacokinetic applications
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Tiwari, Amrendra K.
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Wahajuddin
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[J]. BIOANALYSIS, 2022, 14 (14) : 1005 - 1020

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