LC-MS/MS method for simultaneous estimation of raloxifene, cladrin in rat plasma: application in pharmacokinetic studies

被引：4
作者：
Chauhan, Divya [1 ,2 ]
Dadge, Shailesh [1 ,2 ]
Yadav, Pavan K. [1 ,2 ]
Sultana, Nazneen [1 ]
Agarwal, Arun [1 ,2 ]
Vishwakarma, Sachin [1 ]
Rathaur, Shivam [1 ]
Yadav, Shubhi [1 ]
K Chourasia, Manish [1 ,2 ]
Gayen, Jiaur R. [1 ,2 ]
机构：
[1] Cent Drug Res Inst, Div Pharmaceut & Pharmacokinet, Lucknow 226031, Uttar Pradesh, India
[2] Acad Sci & Innovat Res, Ghaziabad 201002, India
来源：
BIOANALYSIS | 2024年 / 16卷 / 03期
关键词：
bioanalytical method validation; co-estimation; LC-MS/MS; pharmacokinetics; raloxifene and cladrin; simultaneous identification; ESTROGEN-RECEPTOR MODULATORS; UPLC-MS/MS; VALIDATION; OSTEOPOROSIS; METABOLITES; ASSAY;
D O I：
10.4155/bio-2023-0206
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Aim: A newer LC-MS/MS method was developed and validated for the simultaneous quantification of raloxifene (RL) and cladrin (CL). Methodology: Both drugs were resolved in RP-18 (4.6 x 50 mm, 5 mu) Xbridge Shield column using acetonitrile and 0.1% aqueous solution of formic acid (FA) (70:30% v/v) as mobile phase by using biological matrices in female Sprague-Dawley rats using-MS/MS. Results: The developed method was found to be linear over the concentration ranges of 1-600 ng/ml, and lower limit of quantification was 1 ng/ml for RL and CL, respectively. Pharmacokinetic results of RL+CL showed C-max = 4.23 +/- 0.61, 26.97 +/- 1.14 ng/ml, at T-max(h) 5.5 +/- 1.00 and 3.5 +/- 1.00, respectively. Conclusion: Pharmacokinetic study results will be useful in the future for the combined delivery of RL and CL for osteoporosis treatment. [GRAPHICS] .
引用
收藏
页码：141 / 153
页数：14
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