Chloroplast-targeted late embryogenesis abundant 1 increases alfalfa tolerance to drought and aluminum

被引：18

作者：

Lv, Aimin ^{[1
,2
]}

Su, Liantai ^{[1
]}

Fan, Nana ^{[1
]}

Wen, Wuwu ^{[1
]}

Wang, Zheng ^{[1
]}

Zhou, Peng ^{[1
]}

An, Yuan ^{[1
,3
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China

[2] Zhejiang A&F Univ, Zhejiang Prov Key Lab Resources Protect & Innovat, Hangzhou 311300, Peoples R China

[3] Minist Agr, Key Lab Urban Agr, Shanghai 201101, Peoples R China

来源：

PLANT PHYSIOLOGY | 2023年 / 193卷 / 04期

基金：

中国博士后科学基金;

关键词：

LEA PROTEINS; GENE-EXPRESSION; RUBISCO; PHOTOSYNTHESIS; SUBUNIT; COLD;

D O I：

10.1093/plphys/kiad477

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Late embryogenesis-abundant (LEA) proteins are important stress-response proteins that participate in protecting plants against abiotic stresses. Here, we investigated LEA group 3 protein MsLEA1, containing the typically disordered and & alpha;-helix structure, via overexpression and RNA interference (RNAi) approaches in alfalfa (Medicago sativa L.) under drought and aluminum (Al) stresses. MsLEA1 was highly expressed in leaves and localized in chloroplasts. Overexpressing MsLEA1 increased alfalfa tolerance to drought and Al stresses, but downregulating MsLEA1 decreased the tolerance. We observed a larger stomatal aperture and a lower water use efficiency in MsLEA1 RNAi lines compared with wild-type plants under drought stress. Photosynthetic rate, Rubisco activity, and superoxide dismutase (SOD) activity increased or decreased in MsLEA1-OE or MsLEA1-RNAi lines, respectively, under drought and Al stress. Copper/zinc SOD (Cu/Zn-SOD), iron SOD (Fe-SOD), and Rubisco large subunit proteins (Ms1770) were identified as binding partners of MsLEA1, which protected chloroplast structure and function under drought and Al stress. These results indicate that MsLEA1 recruits and protects its target proteins (SOD and Ms1770) and increases alfalfa tolerance against drought and Al stresses. Chloroplast-targeted MsLEA1 increases the tolerance of alfalfa under drought and aluminum stresses via interacting with superoxide dismutase and Rubisco subunit protein.

引用

页码：2750 / 2767

页数：18