LncRNA UCA1 could regulate the progression of neuropathic pain by regulating miR-135a-5p

被引:1
作者
Wu, Bingbing [1 ]
Zhou, Xiaogang [1 ,2 ]
机构
[1] Nantong Univ, Affiliated Hosp 2, Dept Orthopaed, Nantong 226001, Jiangsu, Peoples R China
[2] Nantong Univ, Affiliated Hosp 2, Dept Orthopaed, North Hai Er Xiang Rd 6, Nantong 226001, Jiangsu, Peoples R China
来源
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 2023年 / 827卷
关键词
NPP; UCA1; MiR-135a-5p; Inflammation; AXIS;
D O I
10.1016/j.mrfmmm.2023.111833
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: Neuropathic pain (NPP) is known as a common neurological disease with high incidence rate. The present work focused on the roles of long non-coding RNA urothelial carcinoma antigen 1(LncRNA UCA1) in NPP and the possible underlying mechanism. Methods: NPP rat model has been established and the levels of UCA1 NPP as well as the group has been determined by RT-PCR method. Next, NPP rats were treated by UCA1 over-expression plasmid and the behaviors, as well as expression of inflammatory cytokines have been examined. Furthermore, target miRNA of UCA1, miR135a-5p, has been predicted by bioinformatic method, and further verified with the dual-luciferase reporter assay. Finally, the effects of UCA1/ miR-135a-5p axis have been further evaluated. Results: Expressions of UCA1 were markedly decreased and miR-135a-5p were significantly increased in NPP rats in comparison with the control rats. Over-expression of UCA1 alleviated the inflammatory condition in NPP model by decreasing expression of inflammatory cytokines. miR-135a-5p was confirmed to be a target microRNA of UCA1, and UCA1 may regulate the progress of NPP via targeting miR-135a-5p. Conclusion: UCA1 could regulate NPP via affecting miR-135a-5p expression.
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页数:8
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