Cotton leaf curl Multan virus C4 protein suppresses autophagy to facilitate viral infection

被引:16
作者
Yang, Meng [1 ,2 ]
Ismayil, Asigul [1 ,2 ]
Gao, Teng [1 ,2 ,3 ]
Ye, Zihan [1 ,2 ]
Yue, Ning [1 ,2 ]
Wu, Jie [1 ,2 ]
Zheng, Xiyin [1 ,2 ]
Li, Yiqing [1 ,2 ]
Wang, Yan [1 ,2 ]
Hong, Yiguo [1 ,4 ,5 ]
Liu, Yule [1 ,2 ]
机构
[1] Tsinghua Univ, Ctr Plant Biol, Sch Life Sci, MOE Key Lab Bioinformat, Beijing 100084, Peoples R China
[2] Tsinghua Peking Ctr Life Sci, Beijing 100084, Peoples R China
[3] Beijing Adv Innovat Ctr Struct Biol, Beijing 100084, Peoples R China
[4] Hangzhou Normal Univ, Coll Life & Environm Sci, Res Ctr Plant RNA Signaling, Hangzhou 310036, Peoples R China
[5] Univ Warwick, Sch Life Sci, Warwick Hangzhou RNA Signaling Joint Lab, Warwick CV4 7AL, England
基金
中国国家自然科学基金; 国家重点研发计划;
关键词
REGULATE AUTOPHAGY; CELL-DEATH; GEMINIVIRUS; DETERMINANT; DEGRADATION; INTERACTS; IMMUNITY; DEFENSE;
D O I
10.1093/plphys/kiad235
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Autophagy plays an important role in plant antiviral defense. Several plant viruses are reported to encode viral suppressor of autophagy (VSA) to prevent autophagy for effective virus infection. However, whether and how other viruses, in particular DNA viruses, also encode VSAs to affect viral infection in plants is unknown. Here, we report that the C4 protein encoded by Cotton leaf curl Multan geminivirus (CLCuMuV) inhibits autophagy by binding to the autophagy negative regulator eukaryotic translation initiation factor 4A (eIF4A) to enhance the eIF4A-Autophagy-related protein 5 (ATG5) interaction. By contrast, the R54A or R54K mutation in C4 abolishes its capacity to interact with eIF4A, and neither C4(R54A) nor C4(R54K) can suppress autophagy. However, the R54 residue is not essential for C4 to interfere with transcriptional gene silencing or post-transcriptional gene silencing. Moreover, plants infected with mutated CLCuMuV-C4(R54K) develop less severe symptoms with decreased levels of viral DNA. These findings reveal a molecular mechanism underlying how the DNA virus CLCuMuV deploys a VSA to subdue host cellular antiviral autophagy defense and uphold viral infection in plants. A geminiviral protein suppresses autophagy by enhancing the interaction between the autophagy negative regulator eIF4A and ATG5 to facilitate virus infection.
引用
收藏
页码:708 / 720
页数:13
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