Three-dimensional mouse cochlea imaging based on the modified Sca/eS using confocal microscopy

被引：4
|
作者：
Urata, Shinji [1 ]
Okabe, Shigeo [2 ]
机构：
[1] Univ Tokyo, Grad Sch Med, Dept Otolaryngol, Tokyo 1130033, Japan
[2] Univ Tokyo, Grad Sch Med, Dept Cellular Neurobiol, Tokyo, Japan
来源：
ANATOMICAL SCIENCE INTERNATIONAL | 2023年 / 98卷 / 03期
关键词：
Stria vascularis; Cochlea; Optical tissue clearing; ScaleS; Macrophage; STRIA VASCULARIS; WHOLE-BRAIN; CELL; QUANTIFICATION; ACTIVATION; BARRIER; ORGANS;
D O I：
10.1007/s12565-023-00703-z
中图分类号：
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号：
100101 ;
摘要：
The three-dimensional stria vascularis (SV) and cochlear blood vessel structure is essential for inner ear function. Here, modified Sca/eS, a sorbitol-based optical-clearing method, was reported to visualize SV and vascular structure in the intact mouse cochlea. Cochlear macrophages as well as perivascular-resident macrophage-like melanocytes were detected as GFP-positive cells of the CX3CR1(+/GFP) mice. This study's method was effective in elucidating inner ear function under both physiological and pathological conditions.
引用
收藏
页码：309 / 317
页数：9
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