Chitosan capped-gold nanoparticles as skin penetration enhancer for small molecules: A study in porcine skin

被引：8

作者：

Singpanna, Kanokwan ^{[1
]}

Pornpitchanarong, Chaiyakarn ^{[1
]}

Patrojanasophon, Prasopchai ^{[1
]}

Rojanarata, Theerasak ^{[1
]}

Ngawhirunpat, Tanasait ^{[1
]}

Li, S. Kevin ^{[2
]}

Opanasopit, Praneet ^{[1
,3
]}

机构：

[1] Silpakorn Univ, Fac Pharm, Pharmaceut Dev Green Innovat Grp PDGIG, Nakhon Pathom 73000, Thailand

[2] Univ Cincinnati, Coll Pharm, Div Pharmaceut Sci, Cincinnati, OH 45267 USA

[3] Silpakorn Univ, Fac Pharm, Nakhon Pathom 73000, Thailand

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2023年 / 640卷

关键词：

Chitosan; Gold nanoparticles; Transdermal delivery; Sodium fluorescein; Skin permeation enhancer; TIGHT JUNCTIONS; DELIVERY; STRATEGIES; PERMEATION;

D O I：

10.1016/j.ijpharm.2023.123034

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Skin is considered one of the most convenient sites for drug administration. The present study evaluated the effect of gold nanoparticles stabilized by chitosan (CS-AuNPs) and citrate ions (Ci-AuNPs) on skin permeation of sodium fluorescein (NaFI) and rhodamine b base (RhB) as small model hydrophilic and lipophilic permeants, respectively. CS-AuNPs and Ci-AuNPs were characterized by transmitted electron microscopy (TEM) and dy-namic light scattering (DLS). Skin permeation was investigated using porcine skin with diffusion cells and confocal laser scanning microscopy (CLSM). The CS-AuNPs and Ci-AuNPs were spherical-shaped nanosized particles (38.4 +/- 0.7 and 32.2 +/- 0.7 nm, respectively). The zeta potential of CS-AuNPs was positive (+30.7 +/- 1.2 mV) whereas that of Ci-AuNPs was negative (-60.2 +/- 0.4 mV). The skin permeation study revealed that CS-AuNPs could enhance the permeation of NaFI with enhancement ratio (ER) of 38.2 +/- 7.5, and the effect was superior to that of Ci-AuNPs. CLSM visualization suggested that skin permeation was enhanced by improving the delivery through the transepidermal pathway. However, the permeability of RhB, a lipophilic molecule, was not significantly affected by CS-AuNPs and Ci-AuNPs. Moreover, CS-AuNPs had no cytotoxic toward human skin fibroblast cells. Therefore, CS-AuNPs are a promising skin permeation enhancer of small polar compounds.

引用

页数：9