Field evidences of fluorescent dissolved organic matter (FDOM) as potential fingerprints for agricultural and urban sources in river environment

Field evidences of the fluorescence differences between agricultural and urban river reaches are still lack. In this study, the middle reaches of Danhe River (DH) and Mihe River (MH) in Shouguang, China, were designed as agricultural and urban river reaches, respectively, to compare the the fluorescence differences in disparate river reaches using excitation-emission matrix coupled with parallel factor analysis (EEM-PARAFAC). Three fluorescence components were identified. C1 (Ex/Em=230,255,295 nm/420 nm) was categorized as humic-like fluorophores, C2 (Ex/Em=230,275 nm/330 nm) was recognized as tryptophan-like substances, and C3 (Ex/Em=215 nm/290 nm) was noted as tyrosine-like and phenylalanine-like compounds. The results showed that the FDOM posed significant differences between agricultural and urban river reaches (P < 0.001). The monitoring sites in DH were rich in C2 (1.90 +/- 0.62 Raman Unit (RU), mean +/- standard deviation), and the monitoring sites in MH were rich in C3 (1.32 +/- 0.51 RU). Redundancy analysis revealed that C2 could be regarded as a fluorescence indicator of agricultural sewage in river environment, while C3 was recognized as a fluorescence indicator of domestic sewage in river environment. In conclusion, this study provided field evidences of FDOM as potential fingerprints for agricultural and urban sources in river environment.