Green synthesis of silver nanoparticles using Helianthemum lippii extracts (Hl-NPs): Characterization, antioxidant and antibacterial activities, and study of interaction with DNA

This study's objective was to formulate silver nanoparticles by reducing the extraction of Helianthemum lippii. We looked into how AgNPs attach to deoxyribonucleic acid using cyclic voltammetry and spec-troscopy (UV-Vis) techniques. The DPPH, Reduction Power (RP), and-Carotene Bleaching (BCB) tests have also been used to assess antioxidant activity. The effectiveness of AgNPs against six bacterial strains was also tested. Biosynthesized AgNPs were characterized using UV-Visible and FTIR Spectroscopy, powder-XRD, and EDX technics. The UV-vis spectra at 428 nm validated the outcomes of the synthe-sized AgNPs. The results of SEM, EDX and powder-XRD, examination showed that AgNPs were the crys-tallite cubic, high purity with size ranged from 4.81 to 12.84 nm. Additionally, the produced AgNPs' FTIR spectra revealed the presence of phytoconstituents that served as capping agents. Also, the ex-perimental findings showed that AgNPs had a strong propensity for binding to DNA through electro-static interactions, which is supported by binding energy values. Additionally, it was demonstrated that biosynthesized AgNPs had high antioxidant activity (IC50 values for the DPPH, RP, and BCB assays were 3.16 +/- 0.02 mu g/mL,1.73 +/- 0.01 mu g/mL, and 1.99 +/- 0.04, respectively). Additionally, results indicated an effi-cient antibacterial action of AgNPs with the MIC range from 0.04 to 0.18 mg/mL against six bacterial strains. To conclude, the current study opens avenues that make them candidates for therapy against many cancer disorders. Additionally, they may be used as treatment for other illnesses brought on by oxidative stress and bacteria.(c) 2023 Published by Elsevier B.V.