Absence of Synergism between a Dual-AMP Biogel and Antibiotics Used as Therapeutic Agents for Diabetic Foot Infections

被引：0

作者：

Soares, Rui Silva ^{[1
,2
]}

Gomes, Diana ^{[1
,2
]}

Serrano, Isa ^{[1
,2
]}

Cunha, Eva ^{[1
,2
]}

Tavares, Luis ^{[1
,2
]}

Oliveira, Manuela ^{[1
,2
,3
,4
]}

机构：

[1] Univ Lisbon, Fac Vet Med, CIISA Ctr Interdisciplinary Res Anim Hlth, Ave Univ Tecn, P-1300477 Lisbon, Portugal

[2] Associate Lab Anim & Vet Sci AL4Anim, Ave Univ Tecn, P-1300477 Lisbon, Portugal

[3] cE3c Ctr Ecol Evolut & Environm Changes, Campo Grande 016, P-1749016 Lisbon, Portugal

[4] CHANGE Global Change & Sustainabil Inst, Campo Grande 016, P-1749016 Lisbon, Portugal

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2024年 / 25卷 / 01期

关键词：

antimicrobial peptides; collagen model; nisin A; pexiganan; Pseudomonas aeruginosa; Staphylococcus aureus; RESISTANT STAPHYLOCOCCUS-AUREUS; ANTIMICROBIAL CATIONIC PEPTIDES; IN-VITRO ACTIVITIES; METHICILLIN-RESISTANT; BACTERIAL-INFECTIONS; COMBINATION; PEXIGANAN; BIOFILM; ULCERS; VANCOMYCIN;

D O I：

10.3390/ijms25010407

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Diabetic foot infections (DFIs) are frequently linked to diabetic-related morbidity and death because of the ineffectiveness of conventional antibiotics against multidrug-resistant bacteria. Pexiganan and nisin A are antimicrobial peptides (AMPs), and their application may complement conventional antibiotics in DFI treatment. A collagen 3D model, previously established to mimic a soft-tissue collagen matrix, was used to evaluate the antibacterial efficacy of a guar gum gel containing pexiganan and nisin alone and combined with three antimicrobials toward the biofilms of Staphylococcus aureus and Pseudomonas aeruginosa isolated from infected foot ulcers. Antimicrobials and bacterial diffusion were confirmed by spot-on-lawn and bacterial growth by bacterial count (cfu/mL). Our main conclusion was that the dual-AMP biogel combined with gentamicin, clindamycin, or vancomycin was not able to significantly reduce bacterial growth or eradicate S. aureus and P. aeruginosa DFI isolates. We further reported an antagonism between dual-AMP and dual-AMP combined with antibiotics against S. aureus.

引用

页数：12