Uncovering the effective substances and mechanism of Ipomoea pes-caprae against rheumatoid arthritis using liquid chromatography-mass spectrometry and targeted network pharmacology

被引：7
作者：
Cheng, Xiaoxu [1 ]
Cui, Naiyun [1 ]
Hou, Xiaotao [2 ,4 ]
Pi, Zifeng [1 ,3 ]
Deng, Jiagang [2 ]
Liu, Shu [1 ,5 ]
机构：
[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Lab Chem Biol,Jilin Provincal Key Lab Chinese Med, Changchun, Peoples R China
[2] Guangxi Univ Chinese Med, Coll Pharm, Guangxi Collaborat Innovat Ctr Res Funct Ingredien, Guangxi Key Lab Efficacy Study Chinese Mat Med, Nanning, Peoples R China
[3] Changchun Univ Chinese Med, Coll Pharm, Changchun, Peoples R China
[4] Guangxi Univ Chinese Med, Nanning 530200, Peoples R China
[5] Chinese Acad Sci, Changchun Inst Appl Chem, Changchun 130022, Peoples R China
来源：
JOURNAL OF SEPARATION SCIENCE | 2023年 / 46卷 / 08期
关键词：
Ipomoea pes-caprae; LC-MS; molecular docking; rheumatoid arthritis; targeted network pharmacology; TNF-ALPHA;
D O I：
10.1002/jssc.202200856
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Ipomoea pes-caprae (L.) R. Br (Convolvulaceae) is a commonly used marine traditional Chinese medicine in the southern coastal areas of China. It has been widely used to treat rheumatoid arthritis, but its effective substances and anti-rheumatoid arthritis mechanism remain ambiguous. Hence, in this study, the chemical profile and absorbed ingredients of Ipomoea pes-caprae were elucidated by ultra-performance liquid chromatography-mass spectrometry. Moreover, targeted network pharmacology was used to clarify the mechanism of action of Ipomoea pes-caprae in treating rheumatoid arthritis. Finally, 23 compounds were identified in the aqueous extracts of Ipomoea pes-caprae and 12 absorbed ingredients were detected in rats' plasma. These 12 absorbed ingredients might be the essential effective substances of Ipomoea pes-caprae. The tissue distributions of 3 absorbed ingredients in rats were successfully analyzed. The targeted network pharmacological analysis results indicated that the regulation of inflammatory reaction, immune response, cell proliferation, and apoptosis were the critical mechanism of Ipomoea pes-caprae against rheumatoid arthritis. This study successfully clarified the effective substances and potential mechanisms of Ipomoea pes-caprae in treating rheumatoid arthritis. The results of this research could provide a valuable reference for further scientific research and clinical application.
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页数：11
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