Effects of calcium silicate-based cements on odonto/osteogenic differentiation potential in mesenchymal stem cells

被引：6
作者：
Sismanoglu, Soner [1 ]
Ercal, Pinar [2 ,3 ]
机构：
[1] Altinbas Univ, Fac Dent, Dept Restorat Dent, Istanbul, Turkey
[2] Queen Mary Univ London QMUL, Inst Dent, Ctr Oral & Mmunobiol & Regenerat Med, London, England
[3] Queen Mary Univ London QMUL, Inst Dent, Ctr Oral Clin Res, London, England
来源：
AUSTRALIAN ENDODONTIC JOURNAL | 2023年 / 49卷 / 01期
关键词：
calcium silicates; mesenchymal stem cells; odonto; osteogenic differentiation; pulp capping; vital pulp therapy; MINERAL TRIOXIDE AGGREGATE; PULPAL RESPONSES; BIODENTINE; MTA; CYTOTOXICITY; EXPRESSION;
D O I：
10.1111/aej.12615
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
The objective of this study was to evaluate the biological effects and odonto/osteogenic differentiation potential of Biodentine, NeoMTA Plus and TheraCal LC in tooth germ-derived stem cells (TGSCs). TGSCs were exposed to the material extracts. Biocompatibility was tested with MTS cell proliferation assay. Odonto/osteogenic differentiation was assessed with alkaline phosphatase (ALP) activity and mRNA gene expressions (RUNX2, DSPP and DMP-1). Scanning electronic microscopy/energy-dispersive X-ray (SEM/EDX) analysis and pH analysis were also performed for the materials. Data were evaluated using the one-way ANOVA and Tukey's tests. TGSCs remained viable after 7 days of incubation with all tested materials. Biodentine and NeoMTA Plus showed high ALP activity and increased expression of RUNX2, DSPP and DMP-1 compared to that of TheraCal LC. All materials can induce odonto/osteogenic differentiation of MSCs in various levels. Biocompatibility and odonto/osteogenic differentiation potential of Biodentine and NeoMTA Plus are similar and superior to that of TheraCal LC.
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页码：66 / 74
页数：9
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