Short heat shock has a long-term effect on mesenchymal stem cells' transcriptome

被引:2
作者
Chorev, Ivana Ribarski- [1 ]
Schudy, Gisele [1 ]
Strauss, Carmit [1 ]
Schlesinger, Sharon [1 ]
机构
[1] Hebrew Univ Jerusalem, Robert H Smith Fac Agr & Food & Environm, IL-7610001 Rehovot, Israel
关键词
STROMAL CELLS; STRESS; DIFFERENTIATION; EXPRESSION; CYTOSCAPE; RESPONSES; GROWTH; FATE; MTOR;
D O I
10.1016/j.isci.2023.107305
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The adverse effects of heat stress (HS) on physiological systems are well docu-mented, yet the underlying molecular mechanisms behind it remain poorly under-stood. To address this knowledge gap, we conducted a comprehensive investiga-tion into the impact of HS on mesenchymal stem cells (MSCs), focusing on their morphology, phenotype, proliferative capacity, and fate determination. Our in-depth analysis of the MSCs' transcriptome revealed a significant influence of HS on the transcriptional landscape. Notably, even after a short period of stress, we observed a persistent alteration in cell identity, potentially mediated by the activation of bivalent genes. Furthermore, by comparing the differentially ex-pressed genes following short HS with their transcriptional state after recovery, we identified the transient upregulation of MLL and other histone modifiers, providing a potential mechanistic explanation for the stable activation of bivalent genes. This could be used to predict and modify the long-term effect of HS on cell identity.
引用
收藏
页数:22
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