Single and combined effects of caffeine and salicylic acid on mussel Mytilus galloprovincialis: Changes at histomorphological, molecular and biochemical levels

被引：11

作者：

Afsa, Sabrine ^{[1
]}

De Marco, Giuseppe ^{[2
]}

Cristaldi, Antonio ^{[3
]}

Giannetto, Alessia ^{[2
]}

Galati, Mariachiara ^{[2
]}

Bille, Barbara ^{[2
]}

Conti, Gea Oliveri ^{[3
]}

ben Mansour, Hedi ^{[1
]}

Ferrante, Margherita ^{[3
]}

Cappello, Tiziana ^{[2
]}

机构：

[1] Univ Monastir, Higher Inst Appl Sci & Technol Mahdia, Res Unit Anal Proc Appl Environm APAE, UR17ES32, Monastir 5000, Tunisia

[2] Univ Messina, Dept Chem Biol Pharmaceut & Environm Sci, Viale F Stagno dAlcontres 31, I-98166 Messina, Italy

[3] Univ Catania, Dept Med Surg & Adv Technol GF Ingrassia, Environm & Food Hyg LIAA, I-95123 Catania, Italy

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2023年 / 101卷

关键词：

Pharmaceuticals; Antioxidant defensive mechanisms; Bivalves; Histology; Transcriptional regulation; SUPEROXIDE-DISMUTASE SOD; INTEGRATED BIOMARKER RESPONSE; POLAR ORGANIC MICROPOLLUTANTS; CLAM RUDITAPES-PHILIPPINARUM; OXIDATIVE STRESS BIOMARKERS; GLUTATHIONE S-TRANSFERASES; PERSONAL CARE PRODUCTS; WATER TREATMENT PLANTS; ZEBRAFISH DANIO-RERIO; WASTE-WATER;

D O I：

10.1016/j.etap.2023.104167

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Caffeine (CAF) and salicylic acid (SA) are frequently detected in waterbody, though information on their biological impact is poor. This work assesses the effects of CAF (5 ng/L to 10 & mu;g/L) and SA (0.05 & mu;g/L to 100 & mu;g/L) alone and combined as CAF+SA (5 ng/L+0.05 & mu;g/L to 10 & mu;g/L+100 & mu;g/L) on mussel Mytilus galloprovincialis under 12-days exposure by histomorphology of digestive gland and oxidative stress defense at molecular and biochemical levels. Besides evaluating tissue accumulation, absence of histomorphological damage and haemocyte infiltration highlighted activation of defensive mechanisms. Up-regulation of Cu/Zn-sod, Mn-sod, cat and gst combined with increased catalase and glutathione S-transferase activity were found in CAF-exposed mussels, while SA reduced ROS production and mitochondrial activity. CAF+SA exposure induced differential responses, and the integrated biomarker response (IBR) revealed more pronounced effects of SA than CAF. These results enlarge knowledge on pharmaceuticals impact on non-target organisms, emphasizing the need for proper environmental risk assessment.

引用

页数：19

共 144 条

[1] Toxicological impact of environmental microplastics and benzo[a]pyrene in the seaworm Hediste diversicolor under environmentally relevant exposure conditions [J].